Document Detail


The association between serotonin transporter gene promoter polymorphism (5-HTTLPR), self-reported symptoms, and dental mercury exposure.
MedLine Citation:
PMID:  18686203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The associations between a polymorphism of the serotonin transporter gene (5-HTTLPR), dental mercury exposure, and self-reported symptoms were evaluated among 157 male dentists and 84 female dental assistants. Self-reported symptoms and detailed work histories were obtained by computerized questionnaire. Spot urine samples were collected and analyzed for mercury concentrations to evaluate recent exposures, whereas a chronic mercury exposure index was created from the work histories. 5-HTTLPR polymorphism status was determined using a polymerase chain reaction (PCR)-based assay. Scores for current, recent, and chronic self-reported symptom groups were evaluated with respect to recent and chronic mercury exposure and 5-HTTLPR polymorphism status. Multiple regression analysis controlled for age, socioeconomic status, tobacco and alcohol use, self-reported health problems, and medications. Analyses were restricted to Caucasian subjects due to the highly skewed distribution of the 5-HTTLPR polymorphism. Separate evaluations were conducted for dentists and dental assistants. In contrast to previous reports, no consistent associations were found between either urinary mercury concentration or the chronic index of mercury exposure and any category of symptoms. However, both significant and consistent associations were observed between increased symptoms and the 5-HTTLPR polymorphism involving two copies of the short or "s" allele (full mutation), but not with the polymorphism involving only one copy (heterozygous), demonstrating a gene-dose relationship for symptom reporting. These findings suggest that within this restricted population increased symptoms of depression, anxiety, and memory are associated with the 5-HTTLPR polymorphism among both males and females.
Authors:
Nicholas J Heyer; Diana Echeverria; Federico M Farin; James S Woods
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  71     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2008  
Date Detail:
Created Date:  2008-08-07     Completed Date:  2008-09-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  1318-26     Citation Subset:  IM    
Affiliation:
Battelle Centers for Public Health Research and Evaluation, Seattle, Washington 98109-3598, USA. heyern@battelle.org
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MeSH Terms
Descriptor/Qualifier:
Anxiety / genetics
Cohort Studies
Dental Amalgam / chemistry,  toxicity*
Dental Assistants
Dentists
Depression / genetics
European Continental Ancestry Group
Female
Genetic Predisposition to Disease / genetics*
Health Surveys
Humans
Male
Memory Disorders / genetics
Mercury / urine
Mercury Poisoning, Nervous System / genetics*
Occupational Diseases / genetics
Occupational Exposure / adverse effects*
Polymorphism, Single Nucleotide / genetics*
Serotonin Plasma Membrane Transport Proteins / genetics*
Grant Support
ID/Acronym/Agency:
P30 ES007033-069006/ES/NIEHS NIH HHS; P30 ES007033-06S19006/ES/NIEHS NIH HHS; P30 ES007033-06S29006/ES/NIEHS NIH HHS; P30 ES007033-079006/ES/NIEHS NIH HHS; P30 ES007033-07S19006/ES/NIEHS NIH HHS; P30 ES007033-089006/ES/NIEHS NIH HHS; P30 ES007033-099006/ES/NIEHS NIH HHS; P30 ES007033-109006/ES/NIEHS NIH HHS; P42 ES004696-110002/ES/NIEHS NIH HHS; P42 ES004696-180002/ES/NIEHS NIH HHS; P42 ES004696-190002/ES/NIEHS NIH HHS; P42 ES004696-200002/ES/NIEHS NIH HHS; P42 ES004696-210002/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/SLC6A4 protein, human; 0/Serotonin Plasma Membrane Transport Proteins; 7439-97-6/Mercury; 8049-85-2/Dental Amalgam
Comments/Corrections

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