Document Detail

The association between RhEPO and FN expression in glomerular mesangial cells.
MedLine Citation:
PMID:  22801437     Owner:  NLM     Status:  In-Data-Review    
AIM: Erythropoietin (rhEPO) is increasingly being used in the treatment of anemia caused by miscellaneous reasons. The aim of our research is to investigate the effects of recombinant human erythropoietin (rhEPO) on cellular growth and fibronectin (FN) expression in glomerular mesangial cells.
METHODS: Western blot was used to detect the expression of FN induced by rhEPO (1, 10, 100 and 1000U/mL). In vivo studies, male CD-1 mice were administered rhEPO subcutaneously at a single dose of 1000U/Kg. The cellular hypertrophy was quantified by counting cell number and calculating the ratio of cell protein to cell number.
RESULTS: 1) Compared with the control group, the results of mesangial cells' growth stimulated by rhEPO were not significantly different; 2) RhEPO lead to hypertrophy of mesangial cells; 3) rhEPO induced FN expression of mesangial cell in a dose-dependent way. Compared to control, 100U/mL rhEPO enhanced the expression of FN significantly; 4) indirect immunofluorescence showed that rhEPO induced large amount deposition of FN in the intercellular space of mesangial cells; 5) in vivo studies, there were markedly increase of FN expression in mice received the injection of rhEPO.
CONCLUSION: RhEPO could up-regulated the expression of FN and induced glomerular mesangial cells hypertrophy. These results suggested rhEPO could induce dysfunction of renal glomerulus through its influence on the function of mesangial cells.
S Q Zhou; P Wen; L Fang; L Jiang; M Xiong; F F Zhang; J W Yang
Related Documents :
22889647 - Nanomaterial based self-referencing microbiosensors for cell and tissue physiology rese...
22738617 - Heterogeneity of f cells in β-thalassemia.
7425437 - Hierarchic classification of multiple types of urothelial cells by computer.
22872317 - Syndecan-4 is associated with beta-cells in the pancreas and the min6 beta-cell line.
20596927 - Progress in glial cell studies in some laboratories in china.
23864037 - Intracellular viscoelasticity of hela cells during cell division studied by video parti...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Panminerva medica     Volume:  54     ISSN:  1827-1898     ISO Abbreviation:  Panminerva Med     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421110     Medline TA:  Panminerva Med     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  205-9     Citation Subset:  IM    
Emergency Department and Surgical Intensive Care Unit, Shanghai 10th People's HospitalTongji University School of Medicine. Shanghai, China -
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Vascular burden as a substrate for higher-level gait disorders in older adults. A review of brain ma...
Next Document:  Time course of proadrenomedullin in the early phase of septic shock. A comparative study with other ...