| The assessment of rat ureteral pressure generation in vitro: regional heterogeneity and influence of distending pressure. | |
MedLine Citation:
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PMID: 20843769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contraction of ureteral smooth muscle drives the urine bolus to the urinary bladder for storage prior to micturition. This study describes a novel approach to the measurement of ureteral pressure generation in vitro and the influence of distending pressure on acetylcholine-stimulated ureteral lumenal pressure generation. Isolated segments of ureters obtained from Wistar rats were pressurised in a blind-ended sac arrangement and contractile responses were recorded as phasic oscillations in ureteral luminal pressure. Distal segments generated greater luminal pressures than proximal segments (p<0.001) in response to acetylcholine. Increasing baseline distending pressures in the range 2-10 mmHg in proximal segments was associated with greater frequency of contraction (p<0.001) and decreased magnitude of contraction (p<0.001) when expressed as % maximum response. Nifedipine (10(-5) M) or removal of extracellular Ca(2+) abolished the contractions. Isometric contractile responses of ureteral ring preparations were not significantly influenced by pretensions equivalent to distending pressures in the range 2-10 mmHg. This is the first study to fully establish the influence of baseline ureteral distending pressure upon ureteral luminal pressure generation in vitro and demonstrates regional heterogeneity of ureteral contractile responses. It is suggested that this experimental approach may be a useful methodology for the investigation of ureteral function during urinary outflow obstruction. |
Authors:
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D Rasidovic; D Kearney; Kate-Marie Boyle; S J Bund |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta physiologica Hungarica Volume: 97 ISSN: 0231-424X ISO Abbreviation: Acta Physiol Hung Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-16 Completed Date: 2010-10-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309201 Medline TA: Acta Physiol Hung Country: Hungary |
Other Details:
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Languages: eng Pagination: 307-15 Citation Subset: IM |
Affiliation:
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University College Dublin, UCD School of Medicine and Medical Science, Dublin 4, Ireland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Animals Calcium Channel Blockers / pharmacology Cholinergic Agents / pharmacology Isometric Contraction / drug effects, physiology* Male Muscle, Smooth / physiology* Nifedipine / pharmacology Peristalsis / drug effects, physiology* Pressure Rats Rats, Wistar Ureter / physiology* Urination / physiology |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channel Blockers; 0/Cholinergic Agents; 21829-25-4/Nifedipine; 51-84-3/Acetylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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