| The assessment of brain injury with measurement of calcium fluorescent intensity of a vital brain slice. | |
| | |
MedLine Citation:
|
PMID: 12395964 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Retrograde cerebral perfusion (RCP) is one of cerebral protection methods during deep hypothermic circulatory arrest (DHCA). However, the mechanism is unclear. In this study with laser confocal scanning microscope (LCSM), calcium fluorescent intensity of vital brain slice was compared between RCP and DHCA group. Sixteen swine, weighing from 19 to approximately 20 kg and supplied by Beijing College of Agriculture, were used for the experiment. After 90 min of DHCA or RCP through the superior vena cava, the animals were rewarmed for 120 min. Through tentorium of the cerebellum and enucleation of the eyeball, vital brain slices (cerebellula and retina) were obtained and fluorescein labeled in artificial cerebrospinal fluid containing 5 micromol/L Fluo-3/AM (fluoresence probe). The calcium fluorescent intensity was examined by LCSM. The results indicated that calcium fluorescent intensity of vital brain slice was lower in the RCP group (cerebellula, 9.16 +/- 3.98; retina, 21.48 +/- 14.27) than that in the DHCA group (cerebellula, 31.97 +/- 20.59; retina, 44.07 +/- 21.01) (p < .05). More moderate and severe eosinophilic degeneration was found in the DHCA group than in the RCP group (p < .05) through morphological examination. The statistical analysis also indicated the calcium fluorescent intensity of the vital brain slice was correlated with the level of moderate and severe eosinophilic degeneration of thee neuron (r = 0.86, p < .05). So "calcium overload" contributes to the injury of neuron after DHCA. RCP is able to attenuate "calcium overload," which has the effect of cerebral protection. |
| | |
Authors:
|
Yulong Guan; Peiqing Dong; Caihong Wan; Jing Yang; Meiling He |
Related Documents
:
|
15591744 - Radiofrequency ablation of liver tumors: how to enlarge the necrotic zones? 18976644 - Arylesterase and paraoxonase activity of paraoxonase (pon1) affected by ischemia in the... 10728354 - Preconditioning the human myocardium by simulated ischemia: studies on the early and de... 1444624 - Exercise induced release of von willebrand factor: evidence for hypoxic reperfusion mic... 3545824 - Hormonal regulation of the rate of the glycogen/glucose-1-phosphate cycle in skeletal m... 2287254 - Cardiovascular adjustments to exercise distributed between the upper and lower body. |
Publication Detail:
|
Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of extra-corporeal technology Volume: 34 ISSN: 0022-1058 ISO Abbreviation: J Extra Corpor Technol Publication Date: 2002 Sep |
Date Detail:
|
Created Date: 2002-10-24 Completed Date: 2002-11-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0267637 Medline TA: J Extra Corpor Technol Country: United States |
Other Details:
|
Languages: eng Pagination: 185-9 Citation Subset: T |
Affiliation:
|
Extracorporeal Circulation Department, Beijing Anzhen Hospital, Beijing Capital Medical University, Beijing Heart, Lung, and Blood Vessel Medical Institute, People's Republic of China. guanyulong@yahoo.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aniline Compounds
/
chemistry* Animals Brain Injuries / metabolism, physiopathology*, veterinary Calcium / metabolism* Fluorescent Dyes / chemistry* Microscopy, Confocal Microscopy, Fluorescence Reperfusion / methods*, veterinary Swine Swine Diseases / metabolism, physiopathology* Xanthenes / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Aniline Compounds; 0/Fluorescent Dyes; 0/Xanthenes; 121714-13-4/Fluo-3; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Electronic data management for the Hemochron Jr. Signature coagulation analyzer.
Next Document: Expanding perfusion services through mobile point-of-care coagulation monitoring.