| The assembly of cell-encapsulating microscale hydrogels using acoustic waves. | |
| | |
MedLine Citation:
|
PMID: 21820734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Microscale hydrogels find widespread applications in medicine and biology, e.g., as building blocks for tissue engineering and regenerative medicine. In these applications, these microgels are assembled to fabricate large complex 3D constructs. The success of this approach requires non-destructive and high throughput assembly of the microgels. Although various assembly methods have been developed based on modifying interfaces, and using microfluidics, so far, none of the available assembly technologies have shown the ability to assemble microgels using non-invasive fields rapidly within seconds in an efficient way. Acoustics has been widely used in biomedical arena to manipulate droplets, cells and biomolecules. In this study, we developed a simple, non-invasive acoustic assembler for cell-encapsulating microgels with maintained cell viability (>93%). We assessed the assembler for both microbeads (with diameter of 50 μm and 100 μm) and microgels of different sizes and shapes (e.g., cubes, lock-and-key shapes, tetris, saw) in microdroplets (with volume of 10 μL, 20 μL, 40 μL, 80 μL). The microgels were assembled in seconds in a non-invasive manner. These results indicate that the developed acoustic approach could become an enabling biotechnology tool for tissue engineering, regenerative medicine, pharmacology studies and high throughput screening applications. |
| | |
Authors:
|
Feng Xu; Thomas D Finley; Muge Turkaydin; Yuree Sung; Umut A Gurkan; Ahmet S Yavuz; Rasim O Guldiken; Utkan Demirci |
Related Documents
:
|
11952654 - Management of hip dislocation with postural management. 19422964 - The management of eyelid burns. 12880734 - The wrist in axillary burns--a forgotten focus of concern. 11873544 - Deep partial thickness burn after contact with a meal ready-to-eat heater. 2356164 - Why medical ethics? 6739754 - Radiographers as managers. 4. the organization and its structure--some theory and pract... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-08-06 |
Journal Detail:
|
Title: Biomaterials Volume: 32 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-08-22 Completed Date: 2011-12-19 Revised Date: 2011-12-21 |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 7847-55 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Demirci Bio-Acoustic-MEMS in Medicine Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Survival / drug effects Cells, Immobilized / cytology, drug effects, metabolism Fibroblasts / cytology*, drug effects, metabolism Hydrogels / pharmacology* Mice Microspheres* NIH 3T3 Cells Sound* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 A1081534//PHS HHS; R01 AI081534-01/AI/NIAID NIH HHS; R21 AI087107/AI/NIAID NIH HHS; R21 AI087107-01A1/AI/NIAID NIH HHS; R21 EB007707-01A1/EB/NIBIB NIH HHS; R21 HL095960/HL/NHLBI NIH HHS; R21 HL095960-01A2/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hydrogels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pulse oximetry screening for congenital heart defects in newborn infants (PulseOx): a test accuracy ...
Next Document: Influence of chondroitin sulfate and hyaluronic acid on structure, mechanical properties, and glioma...