Document Detail

FUS/TLS assembles into stress granules and is a prosurvival factor during hyperosmolar stress.
MedLine Citation:
PMID:  23625794     Owner:  NLM     Status:  MEDLINE    
FUsed in Sarcoma/Translocated in LipoSarcoma (FUS/TLS or FUS) has been linked to several biological processes involving DNA and RNA processing, and has been associated with multiple diseases, including myxoid liposarcoma and amyotrophic lateral sclerosis (ALS). ALS-associated mutations cause FUS to associate with stalled translational complexes called stress granules under conditions of stress. However, little is known regarding the normal role of endogenous (non-disease linked) FUS in cellular stress response. Here, we demonstrate that endogenous FUS exerts a robust response to hyperosmolar stress induced by sorbitol. Hyperosmolar stress causes an immediate re-distribution of nuclear FUS to the cytoplasm, where it incorporates into stress granules. The redistribution of FUS to the cytoplasm is modulated by methyltransferase activity, whereas the inhibition of methyltransferase activity does not affect the incorporation of FUS into stress granules. The response to hyperosmolar stress is specific, since endogenous FUS does not redistribute to the cytoplasm in response to sodium arsenite, hydrogen peroxide, thapsigargin, or heat shock, all of which induce stress granule assembly. Intriguingly, cells with reduced expression of FUS exhibit a loss of cell viability in response to sorbitol, indicating a prosurvival role for endogenous FUS in the cellular response to hyperosmolar stress.
Reddy Ranjith K Sama; Catherine L Ward; Laura J Kaushansky; Nathan Lemay; Shinsuke Ishigaki; Fumihiko Urano; Daryl A Bosco
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-07-29     Completed Date:  2013-09-30     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2222-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Cell Death / drug effects
Cell Nucleus / drug effects,  metabolism
Cell Survival / drug effects
Cytoplasmic Granules / drug effects,  metabolism*
HEK293 Cells
HeLa Cells
Hypertonic Solutions / pharmacology*
Methylation / drug effects
RNA-Binding Protein FUS / metabolism*
Sorbitol / pharmacology,  toxicity
Stress, Physiological / drug effects*
Grant Support
Reg. No./Substance:
0/Hypertonic Solutions; 0/RNA-Binding Protein FUS; 506T60A25R/Sorbitol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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