| The assay of glycogen phosphorylase in human blood serum and its application to the diagnosis of myocardial infarction. | |
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MedLine Citation:
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PMID: 1122638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methods are described (a) for the estimation of glycogen phosphorylase activity (EC 2.4.1.1) in human blood serum based on the chemical determination of liberated orthophosphate or on the enzymic determination of glucose 1-phosphate in a coupled assay system and (b) for the electrophoretic separation of isophosphorylases I, II, and III in human. Glycogen phosphorylase activities ranging from 1.5 to 18 mU/ml were found in the serum of patients with acute myocardial infarction. In contrast, no glycogen phosphorylase activity was detected in the serum of healthy persons. The enzyme appears in the serum 4 hours after the onset of the infarction and reaches a maximum after 20 to 30 hours. Acrylamide gel electrophoresis of serum after a myocardial infarction revealed only muscle isophosphorylase I, the isoenzyme characteristic of the heart. No phosphorylase activity was detected in serum of patients with angina pectoris, endocarditis, and uncomplicative congestive heart failure. From these findings it appears that the new serum enzyme test may prove to be a valuable addition to presently existing methods for the early differential diagnosis of acute myocardial infarction. |
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Authors:
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E G Krause; H Will; M Böhm; A Wollenberger |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 58 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1975 Jan |
Date Detail:
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Created Date: 1975-06-27 Completed Date: 1975-06-27 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 145-54 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Clinical Enzyme Tests* Diagnosis, Differential Electrophoresis, Disc Heart Diseases / diagnosis Humans Isoenzymes / blood Methods Myocardial Infarction / diagnosis* Phosphorylases / blood* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 2.4.1.-/Phosphorylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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