Document Detail


An assay for antibodies to thyroid plasma membrane using the isotope-labeled protein A.
MedLine Citation:
PMID:  6897038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An assay for detection of serum IgG binding to thyroid membrane using 125I-Protein A was examined. The test serum is incubated with purified thyroid membranes, and the IgG bound to the membrane is detected by its interaction with 125I-Protein A. Most of the bound IgG is not bound to the TSH receptor, because TSH does not induce any appreciable decrease in the binding of the IgG to thyroid membranes. Increased serum IgG binding to thyroid membrane is found in most patients with Graves' disease and Hashimoto's thyroiditis, but not in patients with thyroid cancer or simple goiter. Many sera with positive binding activity showed positive microsomal antibody. Serum IgG binding to thyroid membrane in Graves' disease correlates neither with LATS activity nor thyroglobulin antibody. This finding suggests that TSH receptor is not involved in the reaction. The assay method is useful for measuring the binding immunoglobulin for thyroid membrane that is frequently increased in autoimmune thyroid disease, and the present data provide further support to the concept that thyroid autoimmune disorders are associated with antibodies to thyroid cell surface components.
Authors:
Y Kajita; M Ishida; T Hachiya; T Miyazaki; M Yoshimura; H Ijichi; Y Ochi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrinologia japonica     Volume:  29     ISSN:  0013-7219     ISO Abbreviation:  Endocrinol. Jpn.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-12-16     Completed Date:  1982-12-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0376546     Medline TA:  Endocrinol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  169-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / immunology*
Graves Disease / immunology
Humans
Immunoglobulin G / analysis*,  immunology
Long-Acting Thyroid Stimulator / metabolism
Staphylococcal Protein A*
Thyroglobulin / immunology
Thyroid Diseases / immunology*
Thyroid Gland / immunology*
Thyroiditis, Autoimmune / immunology
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Staphylococcal Protein A; 9010-34-8/Thyroglobulin; 9034-48-4/Long-Acting Thyroid Stimulator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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