Document Detail


areCBA is an operon in Acinetobacter sp. strain ADP1 and Is controlled by AreR, a sigma(54)-dependent regulator.
MedLine Citation:
PMID:  11114944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The areCBA genes in Acinetobacter sp. strain ADP1, determining growth on benzyl alkanoates, are shown to be transcribed as a single operon and regulated by areR, which encodes a regulatory protein of the NtrC/XylR family. Assays of the Are enzymes and of two insertions of lacZ as a reporter gene have shown that the operon is induced by benzyl acetate, benzyl alcohol, and benzaldehyde, as well as 2- and 4-hydroxybenzyl acetates and benzyl propionate and butyrate. Two adjacent sites of transcriptional initiation were 97 and 96 bp upstream of the start codon for areC, near a sigma(54)-dependent -12, -24 promoter. Inactivation of areR and rpoN (for RNA polymerase sigma(54)) drastically reduced growth rates on the Are substrates and induction of the operon.
Authors:
R M Jones; P A Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  183     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2000-12-29     Completed Date:  2001-01-18     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-9     Citation Subset:  IM    
Affiliation:
School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Acinetobacter / enzymology*,  genetics,  growth & development
Bacterial Proteins / genetics*,  metabolism
Base Sequence
Benzyl Compounds / metabolism
DNA-Binding Proteins*
DNA-Directed RNA Polymerases / genetics,  metabolism*
Esterases / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genes, Regulator / genetics*
Molecular Sequence Data
Operon / genetics*
Oxidoreductases / genetics,  metabolism
RNA Polymerase Sigma 54
Reverse Transcriptase Polymerase Chain Reaction
Sigma Factor / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Benzyl Compounds; 0/DNA-Binding Proteins; 0/Sigma Factor; EC 1.-/Oxidoreductases; EC 2.7.7.6/DNA-Directed RNA Polymerases; EC 2.7.7.6/RNA Polymerase Sigma 54; EC 3.1.-/Esterases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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