Document Detail

An approach to strain improvement and enhanced production of clavulanic acid in Streptomyces clavuligerus.
MedLine Citation:
PMID:  19129630     Owner:  NLM     Status:  MEDLINE    
To develop a strategy for improved production of clavulanic acid (CA), we investigated the effect of using oils on cell growth and CA production during the fermentation of Streptomyces clavuligerus NRRL 3585. In this analysis, triolein, whose fatty acid is oleic acid only, was the best oil source for CA production, but free fatty acids generated from the hydrolysis of oils in a culture broth negatively impacted CA production and cell growth. Hence, we screened for mutants that were resistant to high concentrations of oleic acid. From this screen we identified a mutant S. clavuligerus, OL13, that had a minimum inhibitory concentration (MIC) to oleic acid of 2.1 g/l, much higher than that of S. clavuligerus NRRL 3585, at 0.4 g/l. Not only was cell growth improved, but maximum CA production, at 1,950 mg/l, was approximately 2.0-fold higher than that of the parent strain.
Sae-Jin Kim; Jin-Oh Kim; Chang-Hun Shin; Hye Won Park; Chan-Wha Kim
Publication Detail:
Type:  Journal Article     Date:  2009-01-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-23     Completed Date:  2009-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  160-4     Citation Subset:  IM    
School of Life Sciences and Biotechnology, Korea University.
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MeSH Terms
Clavulanic Acid / biosynthesis*
Fatty Acids, Nonesterified / metabolism
Oleic Acid / metabolism
Streptomyces / growth & development,  metabolism*
Triolein / metabolism
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 112-80-1/Oleic Acid; 122-32-7/Triolein; 58001-44-8/Clavulanic Acid

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