Document Detail


The application of x-ray analysis in the transmission electron analytical microscope (T.E.A.M.) to the quantitative bulk analysis of biological microsamples.
MedLine Citation:
PMID:  988855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper describes: (a)methods for the high and low temperature ashing of biological tissue samples about 10(-4)-10(-5) g in weight; (b)a method of spraying the solubilized ash on to specimen grids which produces droplets of a size convenient for their quantitative analysis in a transmission electron analytical microscope (T.E.A.M.), but without the need to know the actual volume of individual droplets; (c)the construction of standard curves for determining the absolute concentration of Na, Mg, P, S, K, and Ca simultaneously in a given sample. For most of the elements the error in analysis was about 5% or less. It was proposed that the technique is useful for detecting local element-compartments in complex tissues, and thus may be an important aid in the interpretation of the results of point analyses performed on sections taken from adjacent micro-volumes.
Authors:
T W Davies; A J Morgan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  107     ISSN:  0022-2720     ISO Abbreviation:  J Microsc     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  47-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Calcium / analysis
Chlorides / analysis
Electron Probe Microanalysis*
Magnesium / analysis
Microscopy, Electron*
Myocardium / analysis*
Phosphorus / analysis
Potassium / analysis
Sodium / analysis
Sulfur / analysis
X-Rays
Chemical
Reg. No./Substance:
0/Chlorides; 7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium; 7704-34-9/Sulfur; 7723-14-0/Phosphorus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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