| The application of propidium iodide staining to the study of the macronucleus and micronuclei in the suctorian, Heliophrya sp. | |
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MedLine Citation:
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PMID: 2442859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Morphological changes in the macronucleus and micronuclei of the ciliated protozoon Heliophrya chapmani were investigated using the nucleic acid-specific stain propidium iodide. The fluorescence patterns of nuclei observed in propidium iodide preparations correspond well with those observed using more conventional DNA-specific methods, such as the Feulgen stain. The advantages of propidium iodide staining (minimal cell loss during staining, rapidity of the staining process, and the avoidance of cell damage during hydrolysis) make this method a quick and efficient alternative in the cytochemical study of the protozoan nucleus. |
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Authors:
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D P Fox; J M Kuzava; G B Chapman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Stain technology Volume: 62 ISSN: 0038-9153 ISO Abbreviation: Stain Technol Publication Date: 1987 Jul |
Date Detail:
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Created Date: 1987-10-22 Completed Date: 1987-10-22 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0404535 Medline TA: Stain Technol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 217-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / ultrastructure* Ciliophora / growth & development, ultrastructure* Phenanthridines / diagnostic use* Propidium / diagnostic use* Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Phenanthridines; 36015-30-2/Propidium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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