Document Detail


The application of propidium iodide staining to the study of the macronucleus and micronuclei in the suctorian, Heliophrya sp.
MedLine Citation:
PMID:  2442859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphological changes in the macronucleus and micronuclei of the ciliated protozoon Heliophrya chapmani were investigated using the nucleic acid-specific stain propidium iodide. The fluorescence patterns of nuclei observed in propidium iodide preparations correspond well with those observed using more conventional DNA-specific methods, such as the Feulgen stain. The advantages of propidium iodide staining (minimal cell loss during staining, rapidity of the staining process, and the avoidance of cell damage during hydrolysis) make this method a quick and efficient alternative in the cytochemical study of the protozoan nucleus.
Authors:
D P Fox; J M Kuzava; G B Chapman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  62     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-10-22     Completed Date:  1987-10-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  217-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / ultrastructure*
Ciliophora / growth & development,  ultrastructure*
Phenanthridines / diagnostic use*
Propidium / diagnostic use*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Phenanthridines; 36015-30-2/Propidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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