Document Detail


The application of a photon-counting camera in sensitive, bioluminescence-enhanced detection systems for nucleic acid hybridization. Ultrasensitive detection systems for protein blotting and DNA hybridization, III.
MedLine Citation:
PMID:  2754395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A relatively simple, bioluminescence-enhanced detection system for nucleic acid hybridization, using alkaline phosphatase as a label, was described recently (Hauber, R. & Geiger, R. (1988) Nucl. Acid Res. 16, 1213). The principle of detection is as follows: Alkaline phosphatase releases D-luciferin (Photinus pyralis) from D-luciferin-O-phosphate. Liberated D-luciferin reacts with luciferase, ATP and oxygen with light emission. Light produced is measured with a very sensitive photon counting camera system (Argus-100), allowing the visualization and localization of the specifically bound alkaline phosphatase on nitrocellulose sheets. Under non-optimized conditions the limit of detection is at present about 30 pg of pBR322. A sulphonylated nucleotide probe was used for hybridization.
Authors:
R Hauber; R Geiger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical chemistry and clinical biochemistry. Zeitschrift für klinische Chemie und klinische Biochemie     Volume:  27     ISSN:  0340-076X     ISO Abbreviation:  J. Clin. Chem. Clin. Biochem.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-09-05     Completed Date:  1989-09-05     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  7701860     Medline TA:  J Clin Chem Clin Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  361-3     Citation Subset:  IM    
Affiliation:
Abteilung für Klinische Chemie und Klinische Biochemie, Chirurgischen Klinik Innenstadt, Universität München, FRG.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase
Animals
Beetles
Blotting, Western*
Luminescent Measurements
Nucleic Acid Hybridization*
Radiation
Chemical
Reg. No./Substance:
EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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