| The application of a flow cytometric assay for evaluation of phagocytosis of neutrophils. | |
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MedLine Citation:
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PMID: 9241363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phagocytosis and the release of oxidative products generated by the respiratory burst have been studied in vitro under the influence of non-steroidal anti-inflammatory drugs: naproxen and ibuprofen, using phagocytes of peripheral blood from healthy human donors. Phagocytosis was monitored by flow cytometry in order to investigate the uptake of propidium iodide-labelled bacteria (Staphylococcus aureus) by polymorphonuclear leucocytes. In addition, the phagocytic capacity and percentage of killed bacteria was measured in isolated neutrophils using the Pantazis & Kniker method. It was found that naproxen and ibuprofen affect the phagocytic function and hydrogen peroxide production in the examined granulocytes. These methods might be useful in investigations on neutrophil functions. |
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Authors:
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M Zielińska; W Fenrych |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 44 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 1997 |
Date Detail:
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Created Date: 1997-09-09 Completed Date: 1997-09-09 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: POLAND |
Other Details:
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Languages: eng Pagination: 121-5 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Biochemistry, Karol Marcinkowski University of Medical Sciences, Poznań, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Flow Cytometry
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methods* Humans Neutrophil Activation Neutrophils / cytology*, immunology, microbiology Phagocytosis* Staphylococcus aureus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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