Document Detail


An application of duplex PCR for detection of Leptospira spp. and Orientia tsutsugamushi from wild rodents.
MedLine Citation:
PMID:  18806355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Duplex PCR is useful for detecting two different agents from the same specimen. Kidney specimens are the most suitable for detection of Leptospira spp. For Orientia tsutsugamushi, blood clots, spleen, and liver specimens are considered the most suitable. For this study, kidney tissues were the only specimens obtainable for the PCR. Blood clots, spleen, and liver specimens were not available. However, by using the PCR for scrub typhus, O. tsutsugamushi was detected in the kidney of one rodent. This result shows that kidney specimens can be used to detect O. tsutsugamushi using PCR. Further studies will be necessary in order to be able to compare the detection ratio of O. tsutsugamushi using kidney specimens and blood clots, spleen, and liver specimens.
Authors:
Piyada Wangroongsarb; Watcharee Saengsongkong; Wimol Petkanjanapong; Miruntee Mimgratok; Decha Panjai; Wattanapong Wootta; Toshikatsu Hagiwara
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Japanese journal of infectious diseases     Volume:  61     ISSN:  1344-6304     ISO Abbreviation:  Jpn. J. Infect. Dis.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100893704     Medline TA:  Jpn J Infect Dis     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  407-9     Citation Subset:  IM    
Affiliation:
National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi, Thailand. piyada@dms.moph.go.th
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Wild / microbiology*
DNA, Bacterial / analysis,  isolation & purification
Kidney / microbiology*
Leptospira / classification,  genetics,  isolation & purification*
Leptospirosis / microbiology
Orientia tsutsugamushi / genetics,  isolation & purification*
Polymerase Chain Reaction / methods*
Rodent Diseases / microbiology
Rodentia / microbiology*
Scrub Typhus / microbiology
Sensitivity and Specificity
Thailand
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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