Document Detail


The application of cytochemistry to automation.
MedLine Citation:
PMID:  70465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of cytochemical techniques for use with automated instruments introduces a variety of problems not encountered with traditional cytochemical stains prepared for human visualization. This is particularly true for flow-cytophotometers, which require that cells be stained in suspension, making it difficult to remove reagents once they are added to a staining mixture. It is much less a problem for pattern recognition instruments. The major difficulties relate to cell preparation, fixation, staining, controls and interpretation of instrumental data. The new cytochemical techniques or modifications of existing methods that are required for use with such instruments present a fresh challenge to cytochemists. The need for such methods is great and the rewards may be equivalent.
Authors:
L S Kaplow
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Aug 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  990-1000     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Fixatives
Histocytochemistry / methods*
Histological Techniques
Humans
Leukocyte Count / methods*
Photometry / instrumentation*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Fixatives

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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