Document Detail


The application of cell-based label-free technology in drug discovery.
MedLine Citation:
PMID:  18412175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-based assays are an important part of the drug discovery process allowing for interrogation of targets and pathways in a more physiological setting compared to biochemical assays. One of the main hurdles in the cell-based assay field is to design sufficiently robust assays with adequate signal to noise parameters while maintaining the inherent physiology of the pathway or target being investigated. Conventional label and reporter-based cell assays may be more prone to artifacts due to considerable manipulation of the cell either by the label or over-expression of targets or reporter proteins. Cell-based label-free technologies preclude the need for cellular labeling or over-expression of reporter proteins, utilizing the inherent morphological and adhesive characteristics of the cell as a physiologically relevant and quantitative readout for various cellular assays. Furthermore, these technologies utilize non-invasive measurements allowing for time resolution and kinetics in the assay. In this article, we have reviewed the various label-free technologies that are being used in drug discovery settings and have focused our discussion on impedance-based label-free technologies and its main applications in drug discovery.
Authors:
Biao Xi; Naichen Yu; Xiaobo Wang; Xiao Xu; Yama A Abassi
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Biotechnology journal     Volume:  3     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  484-95     Citation Subset:  IM    
Affiliation:
ACEA Biosciences, San Diego, CA 92121, USA. yabassi@aceabio.com
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MeSH Terms
Descriptor/Qualifier:
Biological Assay / methods*
Cell-Free System / physiology*
Drug Delivery Systems / methods*
Drug Design*
Gene Expression Profiling / methods*
Pharmaceutical Preparations / administration & dosage*
Proteome / metabolism
Signal Transduction / drug effects,  physiology*
Staining and Labeling
Chemical
Reg. No./Substance:
0/Pharmaceutical Preparations; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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