| The application of 3D micropatterning of agarose substrate for cell culture and in situ comet assays. | |
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MedLine Citation:
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PMID: 20149429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the fabrication of a 3D micropatterned agarose substrate that enables the culture of single or multiple cells. Patterning was performed on dried agarose using deep UV irradiation leading to 6-microm-deep micropatterns of 25-70 microm in diameter. Cell adhesion was facilitated by the specific grafting of ECM (extra cellular matrix) proteins such as fibronectin into the micropatterns. We show that the pattern size induced the adhesion of one or more cells, thus allowing precise control of the cell number used in the assay, and that cells proliferated similarly as in standard culture conditions. Moreover, cell polarity appeared well preserved on this substrate, so polarized cells like hepatoma HepaRG cells might maintain their differentiation status and act as primary human hepatocytes for hepatotoxicity testing. These 3D patterned culture slides have been successfully used for in situ comet assays and there is evidence that the genotoxic effects of sub-cytotoxic concentrations of drugs could be analyzed in a large number of single HeLa cells. Coupled with the parallel-based design of the 3D micropatterning, which allows automated image analysis, these results strongly indicate that this new cell array system is suitable for high-throughput cytotoxicity and genotoxicity screening applications. |
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Authors:
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Emilie Mercey; Patricia Obe?d; Denise Glaise; Maria-Luisa Calvo-Mu?oz; Christiane Guguen-Guillouzo; Brigitte Fouqu? |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
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Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-23 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3156-65 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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CEA, DSV, IRTSV, Laboratoire Biopuces, Grenoble, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Comet Assay* Extracellular Matrix Proteins / chemistry Fluorescent Dyes Humans Microscopy, Confocal Sepharose / chemistry* Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
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0/Extracellular Matrix Proteins; 0/Fluorescent Dyes; 9012-36-6/Sepharose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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