Document Detail


The apoptotic effect of cordycepin on human OEC-M1 oral cancer cell line.
MedLine Citation:
PMID:  17031645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cordycepin (3'-deoxyadenosine), a pure compound of Cordyceps sinensis, has been illustrated with anti-tumor effects. In the present study, the apoptotic effect of cordycepin on OEC-M1, a human oral squamous cancer cell line, was investigated by morphological observations, cell viability assay, annexin V-FITC analysis and flow cytometry methods. Results demonstrated that the number of rounded-up cell increased as treatment duration of cordycepin (100 microM) increased from 3 to 48 h, and the plasma membrane blebbing could be observed after 12 h treatment. In cell viability assay, cell surviving rate significantly decreased as the dosage and duration of cordycepin treatment increased (P < 0.05). Moreover, phosphatidylserine flipping on cell membrane could be detected with 3, 6 and 12 h cordycepin treatment, which indicated an early apoptotic phenomenon. Furthermore, cell cycle studies illustrated that the percentage of G1 phase cell declined as the dosages of cordycepin increased (10 microM to 5 mM), while the percentages of G2M and subG1 phase cell increased (P < 0.05) in 12, 24 and 48 h cordycepin treatment. These results further confirmed the apoptotic event. In conclusion, cordycepin significantly induced cell apoptotsis in OEC-M1 human oral squamous cancer cells.
Authors:
Wei-Ciao Wu; Jenn-Ren Hsiao; Yu-Yan Lian; Chun-Yu Lin; Bu-Miin Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-10
Journal Detail:
Title:  Cancer chemotherapy and pharmacology     Volume:  60     ISSN:  0344-5704     ISO Abbreviation:  Cancer Chemother. Pharmacol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-20     Completed Date:  2007-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806519     Medline TA:  Cancer Chemother Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  103-11     Citation Subset:  IM    
Affiliation:
Department of Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Cell Line, Tumor
Cell Membrane / drug effects,  metabolism
Cell Shape / drug effects
Cell Survival / drug effects
Deoxyadenosines / pharmacology*
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Flow Cytometry / methods
G1 Phase / drug effects
G2 Phase / drug effects
Gingival Neoplasms / metabolism,  pathology
Humans
Phosphatidylserines / metabolism
Protein Transport / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Deoxyadenosines; 0/Enzyme Inhibitors; 0/Phosphatidylserines; 73-03-0/cordycepin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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