Document Detail


Apicomplexan parasite, Eimeria falciformis, co-opts host tryptophan catabolism for life cycle progression in mouse.
MedLine Citation:
PMID:  22535959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The obligate intracellular apicomplexan parasites, e.g. Toxoplasma gondii and Plasmodium species, induce an IFNγ-driven induction of host indoleamine 2,3-dioxygenase (IDO), the first and rate-limiting enzyme of tryptophan catabolism in the kynurenine pathway. Induction of IDO1 supposedly depletes cellular levels of tryptophan in host cells, which is proposed to inhibit the in vitro growth of auxotrophic pathogens. In vivo function of IDO during infections, however, is not clear, let alone controversial. We show that Eimeria falciformis, an apicomplexan parasite infecting the mouse caecum, induces IDO1 in the epithelial cells of the organ, and the enzyme expression coincides with the parasite development. The absence or inhibition of IDO1/2 and of two downstream enzymes in infected animals is detrimental to the Eimeria growth. The reduced parasite yield is not due to a lack of an immunosuppressive effect of IDO1 in the parasitized IDO1(-/-) or inhibitor-treated mice because they did not show an accentuated Th1 and IFNγ response. Noticeably, the parasite development is entirely rescued by xanthurenic acid, a by-product of tryptophan catabolism inducing exflagellation in male gametes of Plasmodium in the mosquito mid-gut. Our data demonstrate a conceptual subversion of the host defense (IFNγ, IDO) by an intracellular pathogen for progression of its natural life cycle. Besides, we show utility of E. falciformis, a monoxenous parasite of a well appreciated host, i.e. mouse, to identify in vivo factors underlying the parasite-host interactions.
Authors:
Manuela Schmid; Maik J Lehmann; Richard Lucius; Nishith Gupta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-25
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-11     Completed Date:  2012-09-21     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  20197-207     Citation Subset:  IM    
Affiliation:
Department of Molecular Parasitology, Humboldt University, 10115 Berlin, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coccidiosis / genetics,  immunology,  metabolism*
Culicidae / parasitology
Eimeria / genetics,  immunology,  metabolism*
Enzyme Inhibitors / pharmacology
Hypolipidemic Agents / pharmacology
Indoleamine-Pyrrole 2,3,-Dioxygenase / antagonists & inhibitors,  genetics,  immunology*,  metabolism
Interferon-gamma / genetics,  immunology,  metabolism
Mice
Mice, Inbred BALB C
Mice, Knockout
Th1 Cells / immunology,  metabolism*
Tryptophan / genetics,  immunology,  metabolism*
Xanthurenates / pharmacology
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Hypolipidemic Agents; 0/Indoleamine-Pyrrole 2,3,-Dioxygenase; 0/Xanthurenates; 58LAB1BG8J/xanthurenic acid; 73-22-3/Tryptophan; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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