Document Detail

Is antiquitin a mitochondrial Enzyme?
MedLine Citation:
PMID:  19885858     Owner:  NLM     Status:  MEDLINE    
Antiquitin is an aldehyde dehydrogenase involved in the catabolism of lysine. Mutations of antiquitin have been linked with the disease pyridoxine-dependent seizures. While it is well established that lysine metabolism takes place in the mitochondrial matrix, evidence for the mitochondrial localization of antiquitin has been lacking. In the present study, the subcellular localization of antiquitin was investigated using human embryonic kidney HEK293 cells. Three different approaches were used. First, confocal microscopic analysis was carried out on cells transiently transfected with fusion constructs containing enhanced green fluorescent protein with different lengths of antiquitin based on the different potential start codons of translation. Second, immunofluorescence staining was used to detect the localization of antiquitin directly in the cells. Third, subcellular fractionation was carried out and the individual fraction was analyzed for the presence of antiquitin by Western blot and flow cytometric analyses. All the results showed that antiquitin was present not only in the cytosol but also in the mitochondria.
Judy Wei-Yan Wong; Chi-Lung Chan; Wai-Kwan Tang; Christopher Hon-Ki Cheng; Wing-Ping Fong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  109     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  74-81     Citation Subset:  IM    
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
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MeSH Terms
Aldehyde Dehydrogenase / metabolism*
Blotting, Western
Cell Line
Cell Separation
Cytosol / enzymology
Flow Cytometry
Fluorescent Antibody Technique
Microscopy, Confocal
Mitochondria / enzymology*
Reg. No./Substance:
EC protein, human; EC Dehydrogenase

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