Document Detail


An antimicrobial protein of the gut symbiont Bacteroides fragilis with a MACPF domain of host immune proteins.
MedLine Citation:
PMID:  25339613     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Bacteroidales are the most abundant Gram-negative bacteria of the human intestinal microbiota comprising more than half of the bacteria in many individuals. Some of the factors that these bacteria use to establish and maintain themselves in this ecosystem are beginning to be identified. However, ecological competition, especially interference competition where one organism directly harms another, is largely unexplored. To begin to understand the relevance of this ecological principle as it applies to these abundant gut bacteria and factors that may promote such competition, we screened Bacteroides fragilis for the production of antimicrobial molecules. We found that the production of extracellularly secreted antimicrobial molecules is widespread in this species. The first identified molecule, described in this manuscript, contains a membrane attack complex/perforin (MACPF) domain present in host immune molecules that kill bacteria and virally infected cells by pore formation, and mutations affecting key residues of this domain abrogated its activity. This antimicrobial molecule, termed BSAP-1, is secreted from the cell in outer membrane vesicles and no additional proteins are required for its secretion, processing or immunity of the producing cell. This study provides the first insight into secreted molecules that promote competitive interference among Bacteroidales strains of the human gut.
Authors:
Maria Chatzidaki-Livanis; Michael J Coyne; Laurie E Comstock
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Publication Detail:
Type:  Journal Article     Date:  2014-11-14
Journal Detail:
Title:  Molecular microbiology     Volume:  94     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-09     Completed Date:  -     Revised Date:  2014-12-09    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1361-74     Citation Subset:  IM    
Copyright Information:
© 2014 John Wiley & Sons Ltd.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/N16961
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R01 AI081843/AI/NIAID NIH HHS; R01 AI093771/AI/NIAID NIH HHS

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