Document Detail

The antigenic relatedness of proteins from human and simian prostate fluid.
MedLine Citation:
PMID:  2418427     Owner:  NLM     Status:  MEDLINE    
Two-dimensional electrophoretic analysis of human prostate fluid reveals an abundant protein migrating to a molecular weight of 15 kD and an isoelectric point of 5.5. Polyclonal antibodies were raised specifically to microgram quantities of electrophoresed, excised, and eluted PSP15 (prostate secretory protein). Western immunoblot analysis using these antibodies showed they not only react to PSP15, but cross-react with simian prostate and human seminal fluid proteins of similar molecular weights. Two-dimensional gel immunoblots strongly suggest that the seminal protein and PSP15 are the same, thereby providing a more accessible source of the protein. The antibody to the human PSP15 cross-reacted with neither prostate fluid from the ventral lobe of the rat prostate nor the prostate fluid from the beagle dog.
D B Carter; J G Timmins; L D Adams; R W Lewis; J P Karr; M I Resnick; A E Buhl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Prostate     Volume:  6     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  1985  
Date Detail:
Created Date:  1986-03-26     Completed Date:  1986-03-26     Revised Date:  2005-11-03    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-402     Citation Subset:  IM    
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MeSH Terms
Body Fluids / immunology
Carrier Proteins / immunology*
Macaca mulatta
Molecular Weight
Prospective Studies
Prostate / secretion*
Prostatic Secretory Proteins*
Species Specificity
Reg. No./Substance:
0/Carrier Proteins; 0/Epitopes; 0/Prostatic Secretory Proteins; 0/estramustine-binding protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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