Document Detail

The antigenic binding sites of autoantibodies to factor XII in patients with recurrent pregnancy losses.
MedLine Citation:
PMID:  18278180     Owner:  NLM     Status:  MEDLINE    
Recently, numerous studies have suggested an association between factor XII (FXII) deficiency and recurrent pregnancy losses, and between autoantibodies to FXII and recurrent pregnancy losses. Autoantibodies to FXII rather than FXII deficiency may be a risk factor for recurrent pregnancy losses. To know the pathogenesis of autoantibodies to FXII, epitope mapping study was done. Seventeen anti-FXII antibody positive recurrent pregnancy loss patients were chosen for this study. We used synthetic peptides in inhibition and direct binding studies to identify the antigenic binding site of autoantibodies to FXII. Among plasmas from 17 recurrent pregnancy loss patients who were positive for autoantibodies to FXII, 13 patients (76.5%) recognized amino acids 1-30, the amino-terminal heavy chain region that is known as factor XII binding site to platelet glycoprotein Ibalpha.
Akifumi Inomo; Toshitaka Sugi; Yoshimi Fujita; Hidehiko Matsubayashi; Shun-ichiro Izumi; Mikio Mikami
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  99     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-18     Completed Date:  2008-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  316-23     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, Japan.
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MeSH Terms
Abortion, Habitual / immunology*
Autoantibodies / blood*,  metabolism
Binding Sites, Antibody*
Binding, Competitive
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Epitope Mapping*
Factor XII / chemistry,  immunology*,  metabolism
Peptides / metabolism
Protein Binding
Protein Structure, Tertiary
Reg. No./Substance:
0/Autoantibodies; 0/Binding Sites, Antibody; 0/Peptides; 9001-30-3/Factor XII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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