| The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus). | |
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MedLine Citation:
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PMID: 19944712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Four isoforms of actinoporins were isolated in 2002-2004 from the tropical sea anemone Heteractis crispa (=Radianthus macrodactylus). Their potent hemolytic activities and effects on Ehrlich ascites carcinoma bearing mice were also studied. In this study, the individual actinoporin (RTX-A) demonstrated potential cancer-preventive activity at extremely low and non-cytotoxic concentrations. The substance suppressed the malignant transformation of mouse JB6 P(+) Cl41 cells stimulated by epidermal growth factor (EGF) in soft agar with the inhibition of number of the colonies C(50) (INCC(50))=0.034 nM. Actinoporin RTX-A also was shown to inhibit the phenotype expression of HeLa human cancer cells with an INCC(50)=0.03 nM. The cytotoxic effect of RTX-A against JB6 P(+) Cl41 cells and HeLa, THP-1, MDA-MB-231, and SNU-C4 human tumor cell lines was high (IC(50)=0.57, 2.26, 1.11, 30.0 and 4.66 nM), but significantly less than their capacity to suppress tumor cell colony formation or phenotype expression. RTX-A also induced apoptosis and inhibited basal AP-1, NF-kappaB, and p53-dependent transcriptional activity in JB6 Cl41 cells. These results confirmed that actinoporin RTX-A from H. crispa, at least partially, might exhibit cancer-preventive and anticancer cytotoxic properties through the induction of p53-independent apoptosis and inhibition of the oncogenic AP-1 and NF-kappaB nuclear factors activity. |
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Authors:
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Sergey Fedorov; Sergey Dyshlovoy; Margarita Monastyrnaya; Larisa Shubina; Elena Leychenko; Emma Kozlovskaya; Jun-O Jin; Jong-Young Kwak; Ann M Bode; Zigang Dong; Valentin Stonik |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-26 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 55 ISSN: 1879-3150 ISO Abbreviation: Toxicon Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-06-14 Revised Date: 2011-07-27 |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
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Languages: eng Pagination: 811-7 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia. fedorov@piboc.dvo.ru |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology* Cell Line, Tumor Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cnidarian Venoms / pharmacology* Electrophoresis, Polyacrylamide Gel Epidermal Growth Factor / pharmacology Mice Sea Anemones / chemistry* Transcription, Genetic / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA081064-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Cnidarian Venoms; 0/actinoporin RTX-A, Heteractis crispa; 62229-50-9/Epidermal Growth Factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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