Document Detail


The antibacterial efficacy of an aceraceous plant [Shantung maple (Acer truncatum Bunge)] may be related to inhibition of bacterial beta-oxoacyl-acyl carrier protein reductase (FabG).
MedLine Citation:
PMID:  18208374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyphenols, including flavonoids, are the major components of the extracts from aceraceous plants. They possess remarkable antibacterial and antitumour activity. Our study focused on whether the inhibition of the bacterial type II fatty acid synthesis system is the mechanism for the antibacterial effect of the related plant polyphenols. Extracts obtained from the fallen leaves of the Shantung maple (Acer truncatum Bunge) using different solvents, and the related pure compound PGG (1,2,3,4,6-penta-O-galloyl-beta-D-glucose), potently inhibited the FabG (beta-oxoacyl-ACP reductase) steps in the fatty-acid-elongation cycle with the IC(50) values between 0.9 and 7.2 microg/ml. An ethyl acetate extract appeared to inhibit FabG reductase in a mixed manner with NADPH, as did PGG with NADPH, demonstrating that they interfered with the binding of the cofactor to the enzyme. Gram-positive and Gram-negative bacteria and some fungi were used to evaluate the antibacterial abilities of different extract samples. The experiments showed that a higher polyphenol content of the extracts led to a more potent inhibitory capacity against FabG, thus enhancing the antibacterial efficacy.
Authors:
Feng Zhang; Shi-Yun Luo; Yan-Bin Ye; Wen-Hua Zhao; Xu-Guang Sun; Zhi-Qun Wang; Ran Li; Ying-Hui Sun; Wei-Xi Tian; Ying-Xia Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  51     ISSN:  1470-8744     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2008-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  73-8     Citation Subset:  IM    
Affiliation:
Department of Chemical Biology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Acer / chemistry*
Alcohol Oxidoreductases / antagonists & inhibitors*,  chemistry,  isolation & purification
Anti-Bacterial Agents / chemistry,  isolation & purification,  pharmacology*
Binding Sites
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / chemistry,  isolation & purification,  pharmacology*
Enzyme Activation / drug effects
Enzyme Inhibitors / chemistry,  isolation & purification,  pharmacology*
Escherichia coli / drug effects,  enzymology*
Fungi / drug effects
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Inhibitory Concentration 50
Kinetics
Microbial Sensitivity Tests
Plant Leaves / chemistry
Species Specificity
Structure-Activity Relationship
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Drugs, Chinese Herbal; 0/Enzyme Inhibitors; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.1.36/acetoacetyl-CoA reductase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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