Document Detail

Anti-steroidogenic factor ARR19 inhibits testicular steroidogenesis through the suppression of Nur77 transactivation.
MedLine Citation:
PMID:  20472563     Owner:  NLM     Status:  MEDLINE    
ARR19 (androgen receptor corepressor-19 kDa), a leucine-rich protein whose expression is down-regulated by luteinizing hormone and cAMP, is differentially expressed during the development of Leydig cells and inhibits testicular steroidogenesis by reducing the expression of steroidogenic enzymes. However, the molecular events behind the suppression of testicular steroidogenesis are unknown. In the present study, we demonstrate that ARR19 inhibits the transactivation of orphan nuclear receptor Nur77, which is one of the major transcription factors that regulate the expression of steroidogenic enzyme genes in Leydig cells. ARR19 physically interacts with Nur77 and suppresses Nur77-induced promoter activity of steroidogenic enzyme genes including StAR, P450c17, and 3beta-HSD in Leydig cells. Transient transfection and chromatin immunoprecipitation assays revealed that ARR19-mediated reduced expression of steroidogenic enzyme genes was likely due to the interference of SRC-1 recruitment to Nur77 protein on the promoter of steroidogenic enzyme genes. These findings suggest that ARR19 acts as a novel coregulator of Nur77, in turn regulating Nur77-induced testicular steroidogenesis, and may play an important role in the development and function of testicular Leydig cells.
Imteyaz Qamar; Eun-Yeung Gong; Yeawon Kim; Chin-Hee Song; Hyun Joo Lee; Sang-Young Chun; Keesook Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-14
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-08-06     Revised Date:  2011-11-04    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22360-9     Citation Subset:  IM    
Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
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MeSH Terms
Adenoviridae / metabolism
Binding, Competitive
Cell Nucleus / metabolism
Leydig Cells / cytology,  metabolism
Nuclear Receptor Coactivator 1 / metabolism
Nuclear Receptor Subfamily 4, Group A, Member 1 / genetics*
Promoter Regions, Genetic / genetics
Protein Binding
Protein Structure, Tertiary
Protein Transport
Repressor Proteins / chemistry,  metabolism*
Steroid 17-alpha-Hydroxylase / genetics,  metabolism
Steroids / biosynthesis*
Testis / cytology,  metabolism*
Transcriptional Activation / genetics*
Reg. No./Substance:
0/Cmtm2a protein, mouse; 0/Nuclear Receptor Subfamily 4, Group A, Member 1; 0/Repressor Proteins; 0/Steroids; EC 17-alpha-Hydroxylase; EC Receptor Coactivator 1

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