Document Detail


The anti-apoptotic protein G1P3 is overexpressed in psoriasis and regulated by the non-coding RNA, PRINS.
MedLine Citation:
PMID:  20377629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Psoriasis Susceptibility-Related RNA Gene Induced by Stress (PRINS) is a non-coding RNA overexpressed in lesional and non-lesional psoriatic epidermis and induced by stress. Its function in healthy and psoriatic skin is still not known. Here, we report that PRINS regulates G1P3, a gene with anti-apoptotic effects in keratinocytes. siRNA-mediated inhibition of PRINS gene resulted in altered cell morphology and gene expression alterations, as demonstrated in a microarray experiment. One of the genes regulated by PRINS ncRNA was G1P3, an interferon-inducible gene with anti-apoptotic effects in cancer cells. Interestingly, we found that G1P3 was 400-fold upregulated in hyperproliferative lesional and ninefold upregulated in non-lesional psoriatic epidermis compared to healthy epidermis. In vitro, G1P3 protein levels were highest in proliferating keratinocytes and siRNA-mediated downregulation of G1P3 resulted in increased cell apoptosis. These data indicate that G1P3 inhibits spontaneous keratinocyte apoptosis and hence its high expression in psoriatic skin may contribute to the development of psoriatic lesions. We hypothesize that the deregulation of the PRINS ncRNA may contribute to psoriasis and results in decreased sensitivity to spontaneous keratinocyte apoptosis via the regulation of G1P3.
Authors:
Krisztina Szegedi; Eniko Sonkoly; Nikoletta Nagy; Istv?n Bal?zs N?meth; Zsuzsanna Bata-Cs?rgo; Lajos Kem?ny; Attila Dobozy; M?rta Sz?ll
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  19     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-09     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  269-78     Citation Subset:  IM    
Affiliation:
Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary. krisztinaszegedi@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / genetics*
Base Sequence
Cell Differentiation
Cell Proliferation
Cells, Cultured
DNA Primers / genetics
Epidermis / metabolism,  pathology
Gene Expression Profiling
Gene Expression Regulation / drug effects
Hela Cells
Humans
Keratinocytes / metabolism,  pathology
Lymphokines / pharmacology
Mitochondrial Proteins / genetics*,  metabolism
Oligonucleotide Array Sequence Analysis
Psoriasis / genetics*,  metabolism,  pathology
RNA Interference
RNA, Messenger / genetics,  metabolism
RNA, Untranslated / genetics*
Tissue Culture Techniques
Chemical
Reg. No./Substance:
0/DNA Primers; 0/IFI6 protein, human; 0/Lymphokines; 0/Mitochondrial Proteins; 0/PRINS RNA, human; 0/RNA, Messenger; 0/RNA, Untranslated

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