| An anti-apoptotic pattern correlates with multidrug resistance in acute myeloid leukemia patients: a comparative study of active caspase-3, cleaved PARPs, Bcl-2, Survivin and MDR1 gene. | |
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MedLine Citation:
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PMID: 20557671 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone marrow samples of 17 acute myeloid leukemia (AML) patients were analyzed for apoptosis-related markers. The levels of active caspase-3 (aC-3), Bcl-2 and cleaved poly(ADP-ribose) polymerase (cPARP) were measured by flow cytometry and compared with survivin and MDR1 gene expression as defined by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed heterogeneous patterns of intracellular levels of the studied proteins in AML patients: aC-3 (mean 34.6+/-52.5 U/ml), Bcl-2 (mean 3268.4+/-2055.2 U/ml), and cPARPs (mean 24.59+/-29.97 U/ml). Survivin and MDR1 genes were overexpressed in 9 and 10 patients, respectively. Patients with high levels of survivin mRNA showed significantly lower cPARPs (11.8+/-14.3 versus 53.9+/-31.9 U/ml P=0.005) and a tendency towards higher aC-3 (49.3+/-70.0 versus 18.1+/-9.9 U/ml), and MDR1 overexpression (7/9 patients versus 3/8 patients), as well as poorer therapeutic response and survival. Our data support the potential relevance of apoptosis-related markers in AML for further understanding the disease; however, the heterogeneity and complexity of molecular interactions warrants further prospective studies. |
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Authors:
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Margarita L Guenova; Gueorgui N Balatzenko; Vessela R Nikolova; Branimir V Spassov; Spiro M Konstantinov |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hematology (Amsterdam, Netherlands) Volume: 15 ISSN: 1607-8454 ISO Abbreviation: Hematology Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-18 Completed Date: 2010-10-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708388 Medline TA: Hematology Country: England |
Other Details:
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Languages: eng Pagination: 135-43 Citation Subset: IM |
Affiliation:
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Blood Diseases Diagnostic Unit, Laboratory of Haematopathology and Immunology, National Specialised Hospital for Active Treatment of Haematological Diseases, Sofia 1756, Bulgaria. margenova@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Apoptosis / genetics* Caspase 3 / genetics*, metabolism Drug Resistance, Multiple Drug Resistance, Neoplasm Female Gene Expression Regulation, Neoplastic Humans Immunophenotyping Leukemia, Myeloid, Acute / drug therapy, genetics*, metabolism, pathology Male Microtubule-Associated Proteins / genetics*, metabolism Middle Aged P-Glycoprotein / genetics*, metabolism Poly(ADP-ribose) Polymerases / genetics*, metabolism Proto-Oncogene Proteins c-bcl-2 / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/ABCB1 protein, human; 0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/P-Glycoprotein; 0/Proto-Oncogene Proteins c-bcl-2; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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