Document Detail


The anti-EGFR antibody cetuximab sensitizes human head and neck squamous cell carcinoma cells to radiation in part through inhibiting radiation-induced upregulation of HIF-1α.
MedLine Citation:
PMID:  22348829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we investigated the mechanisms underlying cetuximab-mediated radiosensitization of HNSCC. Irradiation of HNSCC cells upregulated hypoxia-inducible factor-1 alpha (HIF-1α) via a mechanism involving de novo synthesis of HIF-1α protein. Radiation-induced upregulation of HIF-1α was completely abolished by concurrent treatment of HNSCC cells with cetuximab. Experimental elevation of constitutively expressed HIF-1α abolished cetuximab-mediated radiosensitization in HNSCC cells, whereas downregulation of HIF-1α by siRNA or a small molecule inhibitor enhanced responses of cetuximab-resistant HNSCC cells to cetuximab plus radiation. Our data suggest that cetuximab sensitizes cancer cells to ionizing radiation in part through inhibition of radiation-induced upregulation of HIF-1α.
Authors:
Haiquan Lu; Ke Liang; Yang Lu; Zhen Fan
Related Documents :
8392979 - Protein phosphatases limit tumor motility.
18090319 - The tropism of embryoid body cells for glioma cells.
1400639 - Mechanisms of epibolic tissue spreading analyzed in a model morphogenetic system. roles...
8387029 - Differanisole a, an inducer of the differentiation of friend leukemic cells, induces st...
10411959 - Computer-assisted morphometry of cell-substratum contacts.
20807799 - The recruitment of acetylated and unacetylated tropomyosin to distinct actin polymers p...
23988849 - Gold(iii) complexes with esters of cyclohexyl-functionalized ethylenediamine-n,n'-diace...
1366909 - Electrical effects on the proliferation of living hela cells cultured on optically tran...
3955419 - Selective modifications of cellular proteins in intratumoral subpopulations of human co...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-02-17
Journal Detail:
Title:  Cancer letters     Volume:  322     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-05-28     Completed Date:  2012-08-03     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  78-85     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / pharmacology*
Antibodies, Monoclonal, Humanized
Antineoplastic Agents / pharmacology*
Carcinoma, Squamous Cell / radiotherapy*
Cell Line, Tumor
Genes, ras
Head and Neck Neoplasms / radiotherapy*
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / antagonists & inhibitors*,  genetics,  physiology
RNA, Small Interfering / genetics
Radiation Tolerance
Radiation-Sensitizing Agents / pharmacology*
Receptor, Epidermal Growth Factor / antagonists & inhibitors*
Up-Regulation
Grant Support
ID/Acronym/Agency:
CA016672/CA/NCI NIH HHS; P30 CA016672/CA/NCI NIH HHS; R01 CA129036/CA/NCI NIH HHS; R01 CA129036/CA/NCI NIH HHS; R01 CA129036-04/CA/NCI NIH HHS; R21 DE021883/DE/NIDCR NIH HHS; R21 DE021883/DE/NIDCR NIH HHS; R21 DE021883-01/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Monoclonal, Humanized; 0/Antineoplastic Agents; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/RNA, Small Interfering; 0/Radiation-Sensitizing Agents; EC 2.7.10.1/Receptor, Epidermal Growth Factor; PQX0D8J21J/cetuximab
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Design, synthesis, theoretical calculations and biological evaluation of new non-symmetrical choline...
Next Document:  Endoscopic stenting versus gastrojejunostomy for palliation of malignant gastric outlet obstruction.