Document Detail


The anthracenedione compound bostrycin induces mitochondria-mediated apoptosis in the yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  20345898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bostrycin is an anthracenedione with phytotoxic and antibacterial activity that belongs to the large family of quinones. We have isolated bostrycin from the secondary metabolites of a mangrove endophytic fungus, no. 1403, collected from the South China Sea. Using the yeast Saccharomyces cerevisiae as a model, we show that bostrycin inhibits cell proliferation by blocking the cell cycle at G1 phase and ultimately leads to cell death in a time- and dose-dependent manner. Bostrycin-induced lethal cytotoxicity is accompanied with increased levels of intracellular reactive oxygen species and hallmarks of apoptosis such as chromatin condensation, DNA fragmentation and externalization of phosphatidylserine. We further show that bostrycin decreases mitochondrial membrane electric potential and causes mitochondrial destruction during the progression of cell death. Bostrycin-induced cell death was promoted in YCA1 null yeast strain but was partially rescued in AIF1 null mutant both in fermentative and respiratory media, strongly indicating that bostrycin induces apoptosis in yeast cells through a mitochondria-mediated but caspase-independent pathway.
Authors:
Chunling Xu; Jiafeng Wang; Ye Gao; Huangyu Lin; Lin Du; Shanshan Yang; Simei Long; Zhigang She; Xiaoling Cai; Shining Zhou; Yongjun Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-03
Journal Detail:
Title:  FEMS yeast research     Volume:  10     ISSN:  1567-1364     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  297-308     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Anthraquinones / isolation & purification,  toxicity*
Antifungal Agents / isolation & purification,  toxicity*
Apoptosis*
Caspases / deficiency,  metabolism
Cell Cycle / drug effects*
China
Chromatin / metabolism
Culture Media / chemistry
DNA Fragmentation
Membrane Potential, Mitochondrial / drug effects
Microbial Viability / drug effects
Mitochondria / drug effects*
NADH, NADPH Oxidoreductases / deficiency,  metabolism
Phosphatidylserines / metabolism
Saccharomyces cerevisiae / drug effects*,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins / metabolism
Chemical
Reg. No./Substance:
0/Anthraquinones; 0/Antifungal Agents; 0/Chromatin; 0/Culture Media; 0/Phosphatidylserines; 0/Saccharomyces cerevisiae Proteins; 21879-81-2/bostrycin; EC 1.6.-/NADH, NADPH Oxidoreductases; EC 1.6.-/YNR074C protein, S cerevisiae; EC 3.4.22.-/Caspases; EC 3.4.22.-/MCA1 protein, S cerevisiae

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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