Document Detail

An animal model for open femur fracture and osteomyelitis--Part II: Immunomodulation with systemic IL-12.
MedLine Citation:
PMID:  19623664     Owner:  NLM     Status:  MEDLINE    
Infection resulting from open fracture is a common problem in orthopedics. The purpose of this project was to study the effect of Interleukin-12 (IL-12) systemic therapy on a previously established open fracture model. One hundred seven male Sprague-Dawley rats were assigned to five groups: (1) normal (baseline), (2) control (controlled for anesthesia), (3) fracture, (4) staph, and (5) staph and IL-12 (SIL). Each group was divided into four time periods: 6, 10, 14, and 21 days after injury and fixation. The operative groups had a standardized femur fracture and fixation using a Kirschner wire as an intramedullary device. The two infection groups (staph and SIL) were inoculated with Staphylococcus aureus following fracture and fixed with an identical technique. The SIL group was treated with systemic IL-12 for a total of 10 doses over 10 days. Significantly decreased serum IL-12 levels were noted at day 10 in the operative groups compared to the normal and control groups. The SIL group showed significantly higher macrophage activation levels and total platelet counts at day 21 compared to all the other groups. The overall infection rate was not changed by IL-12 supplementation; however, bacterial qualitative growth scores were significantly lower in the SIL group at day 10, which corresponded to the lowest level of systemic IL-12 in the fracture group.
Brock A Lindsey; Nina B Clovis; E Suzanne Smith; Sydha Salihu; David F Hubbard
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  28     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  43-7     Citation Subset:  IM    
Department of Orthopaedics, West Virginia University, P.O. Box 9196 Health Sciences Center, Morgantown, West Virginia 26506-9196, USA.
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MeSH Terms
Adjuvants, Immunologic / administration & dosage,  blood
Disease Models, Animal*
Femoral Fractures / blood,  microbiology*,  therapy
Fracture Fixation, Intramedullary / adverse effects*
Fracture Healing / drug effects
Fractures, Open / blood,  microbiology*,  therapy
Immunomodulation / drug effects
Interleukin-12 / administration & dosage,  blood
Osteomyelitis / blood,  microbiology*,  prevention & control
Rats, Sprague-Dawley
Staphylococcal Infections / blood,  microbiology,  prevention & control
Staphylococcus aureus / drug effects
Surgical Wound Infection / blood,  microbiology*,  prevention & control
Grant Support
Reg. No./Substance:
0/Adjuvants, Immunologic; 187348-17-0/Interleukin-12

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