| Animal model of ascending aortic stenosis in rabbits without endotracheal intubation. | |
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MedLine Citation:
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PMID: 20492033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIM: Experimental animal studies of left ventricular hypertrophy (LVH) usually involve endotracheal intubation, which is associated with a risk of serious tracheal injury and other significant negative sequelae. We developed an animal model of pressure overload hypertrophy caused by constriction of the ascending aorta in rabbits that does not require endotracheal intubation. METHODS: New Zealand White rabbits of either sex (1.94 +/- 0.12 kg) were randomly assigned to the aortic banding (AB) group (n = 9) and the sham-operated group (n = 8). The sternum was carefully incised along the midline to avoid injury to the parietal pleura. Then, the intervention group underwent AB with three to zero Prolene proximal to the innominate artery without endotracheal intubation. To investigate the effects of the surgical procedure on physiological parameters, echocardiography, histology, and electron microscopy were performed to assess functional and structural hypertrophy at various time intervals. RESULTS: Banding of the ascending aorta created the expected increases in both aortic velocity and the pressure gradient between proximal and distal aorta coarctation. The pressure overload resulted in a robust LVH assessed by transthoracic echocardiography and histology. The animals did not experience severe mechanical ventilatory impairment. CONCLUSION: We developed a safe, efficient, and reproducible method of producing LVH in rabbits without the need for endotracheal intubation and mechanical ventilation. Ultimately, this model will facilitate focused study of the mechanisms involved with LVH progression. |
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Authors:
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Zhe Zhao; Lin Chen; Ying-Bin Xiao; Shun-Ji Gao; De-Zhi Zheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cardiac surgery Volume: 25 ISSN: 1540-8191 ISO Abbreviation: J Card Surg Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-05-24 Completed Date: 2010-09-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8908809 Medline TA: J Card Surg Country: United States |
Other Details:
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Languages: eng Pagination: 253-7 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Surgery, Second affiliated Hospital, Third Military Medical University, Chongqing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta* Aortic Valve Stenosis* Disease Models, Animal* Female Hypertrophy, Left Ventricular* Intubation, Intratracheal* Male Rabbits* Respiration, Artificial |
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