Document Detail


The anaphase-promoting complex/cyclosome is required for anaphase progression in multinucleated Ashbya gossypii cells.
MedLine Citation:
PMID:  17158735     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regulated protein degradation is essential for eukaryotic cell cycle progression. The anaphase-promoting complex/cyclosome (APC/C) is responsible for the protein destruction required for the initiation of anaphase and the exit from mitosis, including the degradation of securin and B-type cyclins. We initiated a study of the APC/C in the multinucleated, filamentous ascomycete Ashbya gossypii to understand the mechanisms underlying the asynchronous mitosis observed in these cells. These experiments were motivated by previous work which demonstrated that the mitotic cyclin AgClb1/2p persists through anaphase, suggesting that the APC/C may not be required for the division cycle in A. gossypii. We have now found that the predicted APC/C components AgCdc23p and AgDoc1p and the targeting factors AgCdc20p and AgCdh1p are essential for growth and nuclear division. Mutants lacking any of these factors arrest as germlings with nuclei blocked in mitosis. A likely substrate of the APC/C is the securin homologue AgPds1p, which is present in all nuclei in hyphae except those in anaphase. The destruction box sequence of AgPds1p is required for this timed disappearance. To investigate how the APC/C may function to degrade AgPds1p in only the subset of anaphase nuclei, we localized components and targeting subunits of the APC/C. Remarkably, AgCdc23p, AgDoc1p, and AgCdc16p were found in all nuclei in all cell cycle stages, as were the APC/C targeting factors AgCdc20p and AgCdh1p. These data suggest that the AgAPC/C may be constitutively active across the cell cycle and that proteolysis in these multinucleated cells may be regulated at the level of substrates rather than by the APC/C itself.
Authors:
Amy S Gladfelter; Nicoleta Sustreanu; A Katrin Hungerbuehler; Sylvia Voegeli; Virginie Galati; Peter Philippsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-12-08
Journal Detail:
Title:  Eukaryotic cell     Volume:  6     ISSN:  1535-9778     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-09     Completed Date:  2007-03-29     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  182-97     Citation Subset:  IM    
Affiliation:
University of Basel Biozentrum, Molecular Microbiology, Klingelbergstrasse 0/70, 4056 Basel, Switzerland. Amy.Gladfelter@dartmouth.edu
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MeSH Terms
Descriptor/Qualifier:
Anaphase / physiology*
Ascomycota / cytology*,  genetics,  metabolism
Cell Cycle
Cell Nucleus / metabolism
Computational Biology
Fluorescent Antibody Technique
Fungal Proteins / metabolism
Mitosis
Plasmids
Proteasome Endopeptidase Complex
Ubiquitin-Protein Ligase Complexes / genetics,  metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Fungal Proteins; EC 3.4.25.1/Proteasome Endopeptidase Complex; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes; EC 6.3.2.19/anaphase-promoting complex
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