Document Detail


The analysis of low levels of gamma-glutamyltransferase activity by high-performance liquid chromatography with electrochemical detection.
MedLine Citation:
PMID:  2870663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the analysis of low levels of the enzyme gamma-glutamyltransferase in biological samples by high-performance liquid chromatography with electrochemical detection is described. A gamma-glutamyl moiety from glutathione is transferred by the enzyme to glycylglycine to produce a tripeptide which is assayed directly after a purification step using octadecylsilica. Confirmation of the method is by use of the inhibitor AT-125. The method is used to measure the level of enzyme activity in rodent tissues and in cultured cells.
Authors:
J A Hoskins; F F Davies
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  152     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-05-02     Completed Date:  1986-05-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid / methods*
Electrochemistry
Liver / enzymology
Mice
Oligopeptides
Rats
gamma-Glutamyltransferase / analysis*
Chemical
Reg. No./Substance:
0/Oligopeptides; 13640-39-6/gamma-glutamyl-glycyl-glycine; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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