| An analysis of the isoenzymes of creatine phosphokinase and lactic dehydrogenase in the esophagus. | |
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MedLine Citation:
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PMID: 7283514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Surgical manipulation of muscular organs can cause alterations of the serum isoenzymes of creatine phosphokinase (CPK) and lactic dehydrogenase (LDH), which are frequently used to confirm the diagnosis of myocardial infarction (MI). Since the content of these enzymes and their isoenzymes has not been established for the esophagus, an experiment was conducted to evaluate and compare the enzymes in postmortem specimens from humans and fresh canine specimens. One gram transmural esophageal sections were taken from specimens having no demonstrable disease. All samples were homogenized individually in Ringer's lactate solution and centrifuged, and the supernatants were analysed for the respective isoenzyme distributions by agarose gel electrophoresis. From the study we drew the following conclusions: (1) all three isoenzymes of CPK (including CPK-MB, the myocardialisoenzyme) are present in the esophagus; (2) LDH, the isoenzyme of LDH most prevalent in myocardium, is the least common of the five isoenzymes of LDH in the esophagus; (3) the dog is an appropriate model for studying changes of these isoenzymes after operation; and (4) any potential confusion in diagnosing postoperative MI due to esophageal CPK-MB in the serum can be resolved, theoretically, by analyzing LDH serum isoenzymes. In myocardial infarction, LDH becomes the predominant isoenzyme, whereas esophageal injury should be associated, theoretically, with a serum LDH isoenzyme pattern in which LDH is the least prevalent isoenzyme. |
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Authors:
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G M Graeber; M J Reardon; A W Fleming; H D Head; R Zajtchuk; W H Brott; J H Foster |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 32 ISSN: 0003-4975 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 1981 Sep |
Date Detail:
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Created Date: 1981-11-18 Completed Date: 1981-11-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 230-4 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Creatine Kinase / analysis* Diagnosis, Differential Dogs Electrophoresis, Agar Gel Esophagus / enzymology*, surgery Humans Isoenzymes L-Lactate Dehydrogenase / analysis* Male Myocardial Infarction / diagnosis |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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