Document Detail

An analysis of five serine transfer ribonucleic acids from Drosophila.
MedLine Citation:
PMID:  803967     Owner:  NLM     Status:  MEDLINE    
Crude tRNA from adult Drosophila melanogaster was fractionated on bensoylated-diethylaminoethyl cellulose columns. The eluate was assayed for both amino acid acceptance and cytokinin activity. Most of the cytokinin activity was associated with a peak of serine acceptance. The five major serine tRNAs were purified by chromatography on benzoylated-dietyhlaminoethyl cellulose and reversed phase chromatography-5 columns. The major species, tRNA7-Ser was isolated from this tRNA and was shown to be N-6-(delta-2-isopentenyl)adenosine (i-6A) on the basis of ultraviolet and mass spectral data. The nucleoside somposition of all five serine tRNAs was determined directly and by the 3-H derivative method. They all contain pseudouridine, ribothymidine, 1-methyladenosine, 5-methylcytosine, N-2-dimethylguanosine, 5, 6-hydrouridine, and 3-methylcytosine, while two contain an unidentified nucleoside, and one containes 1-methylguanosine. These techniques also confirmed the presence of i-6A in tRNA7-Ser as well as showing its presence in tRNA6-Ser and tRNA4-Ser. These three tRNA-Ser species exhibit marked changes in elution from reversed phase chromatography-5 columns as a function of temperature and this may be related to their minor base composition. The tRNAs-Ser were bound to ribosomes in response to the following triplets: tRNA2-Ser, AGU, AGC; tRNA4-Ser, UCG; tRNA5-Ser, AGU, AGC; tRNA7-Ser, UCG.
B N White; R Dunn; I Gillam; G M Tener; D J Armstrong; F Skoog; C R Frihart; N J Leonard
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  250     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-06-02     Completed Date:  1975-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  515-21     Citation Subset:  IM    
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MeSH Terms
Adenosine / analogs & derivatives,  analysis
Base Sequence
Chromatography, Ion Exchange
Drosophila melanogaster / genetics*
Mass Spectrometry
RNA, Transfer* / analysis,  isolation & purification
Ribonucleosides / analysis
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Ribonucleosides; 56-45-1/Serine; 58-61-7/Adenosine; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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