| An analysis of five serine transfer ribonucleic acids from Drosophila. | |
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MedLine Citation:
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PMID: 803967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Crude tRNA from adult Drosophila melanogaster was fractionated on bensoylated-diethylaminoethyl cellulose columns. The eluate was assayed for both amino acid acceptance and cytokinin activity. Most of the cytokinin activity was associated with a peak of serine acceptance. The five major serine tRNAs were purified by chromatography on benzoylated-dietyhlaminoethyl cellulose and reversed phase chromatography-5 columns. The major species, tRNA7-Ser was isolated from this tRNA and was shown to be N-6-(delta-2-isopentenyl)adenosine (i-6A) on the basis of ultraviolet and mass spectral data. The nucleoside somposition of all five serine tRNAs was determined directly and by the 3-H derivative method. They all contain pseudouridine, ribothymidine, 1-methyladenosine, 5-methylcytosine, N-2-dimethylguanosine, 5, 6-hydrouridine, and 3-methylcytosine, while two contain an unidentified nucleoside, and one containes 1-methylguanosine. These techniques also confirmed the presence of i-6A in tRNA7-Ser as well as showing its presence in tRNA6-Ser and tRNA4-Ser. These three tRNA-Ser species exhibit marked changes in elution from reversed phase chromatography-5 columns as a function of temperature and this may be related to their minor base composition. The tRNAs-Ser were bound to ribosomes in response to the following triplets: tRNA2-Ser, AGU, AGC; tRNA4-Ser, UCG; tRNA5-Ser, AGU, AGC; tRNA7-Ser, UCG. |
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Authors:
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B N White; R Dunn; I Gillam; G M Tener; D J Armstrong; F Skoog; C R Frihart; N J Leonard |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 250 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1975 Jan |
Date Detail:
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Created Date: 1975-06-02 Completed Date: 1975-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 515-21 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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analogs & derivatives,
analysis Animals Base Sequence Chromatography, Ion Exchange Drosophila melanogaster / genetics* Mass Spectrometry RNA, Transfer* / analysis, isolation & purification Ribonucleosides / analysis Serine* Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Ribonucleosides; 56-45-1/Serine; 58-61-7/Adenosine; 9014-25-9/RNA, Transfer |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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