Document Detail


An analysis of factors for the differential staining of sister chromatids in human chromosomes using Giemsa.
MedLine Citation:
PMID:  87099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various reagents were tested for the purpose of developing an improved Giemsa staining technique for the differential staining of sister chromatids in human chromosomes. Reagents like acids, bases, buffers, protein denaturants and proteolytic enzymes were all potent inducers of differential staining. The best results were obtained by brief trypsinization followed by extraction of nucleic acids by incubation in hot HCl. There was poor contrast between unifilarly and bifilarly BrdU substituted chromatids in slides from which trypsin treatment was omitted. The method of slide preparation as they affect the spreads of BrdU substituted metaphases were also evaluated. The results support the role of these reagents in the conformational changes and structural lesions of chromosomal protein leading to differential staining.
Authors:
R Nand; P K Ghosh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta geneticae medicae et gemellologiae     Volume:  27     ISSN:  0001-5660     ISO Abbreviation:  Acta Genet Med Gemellol (Roma)     Publication Date:  1978  
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370314     Medline TA:  Acta Genet Med Gemellol (Roma)     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Azure Stains
Chromatids*
Chromosomes, Human / ultrastructure*
Humans
Hydrogen-Ion Concentration
Lymphocytes / ultrastructure
Nucleoproteins
Protein Conformation
Staining and Labeling / methods
Trypsin
Chemical
Reg. No./Substance:
0/Azure Stains; 0/Nucleoproteins; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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