| An anaesthetic protocol in the young domestic pig allowing neuromuscular blockade for studies of cardiac function following cardioplegic arrest and cardiopulmonary bypass. | |
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MedLine Citation:
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PMID: 15352961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Neuromuscular blockade should, for ethical reasons, not be allowed in animal experiments unless the use is strongly motivated. Beforehand, the anaesthetic protocol must be documented without muscle relaxation in the species studied. Documentation is difficult to obtain from the scientific literature. When focusing on cardiac function over time, in particular, the ideal anaesthetic protocol should cause no or minor alterations in cardiac variables. METHODS: We intended to document an anaesthetic protocol involving ventilation with N(2)O combined with loading doses and continuous infusions of pentobarbital, fentanyl and midazolam in seven pigs by applying potentially painful stimuli every 15 min for 7 h. Subsequently, left ventricular global and regional function was studied with conductance catheter and strain rate imaging by echocardiography in eight pigs with pancuronium included. RESULTS: Pigs without pancuronium were completely immobilized and unresponsive to potentially painful stimuli and sternotomy, with no accumulation or degradation of anaesthetic agents. With pancuronium included, left ventricular preload gradually decreased together with reduction of cardiac index from 3.52 +/- 0.14 at 2 h to 2.84 +/- 0.11 L min(-1). m(-2) (+/-SEM) after 7 h of observation. Preload recruitable stroke work decreased after 7 h, whereas peak systolic strain in the anterior left ventricular wall and load-independent indices of diastolic function were not significantly altered. CONCLUSION: In specific experimental protocols, the anaesthetic protocol described could allow the use of muscular paralysis in young domestic pigs, for instance when involving hypothermic cardiopulmonary bypass, cardioplegic arrest and reperfusion. |
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Authors:
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T Fanneløp; G O Dahle; K Matre; L Segadal; K Grong |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta anaesthesiologica Scandinavica Volume: 48 ISSN: 0001-5172 ISO Abbreviation: Acta Anaesthesiol Scand Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-08 Completed Date: 2004-12-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370270 Medline TA: Acta Anaesthesiol Scand Country: Denmark |
Other Details:
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Languages: eng Pagination: 1144-54 Citation Subset: IM |
Affiliation:
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Surgical Research Laboratory, Department of Surgical Sciences, University of Bergen, Bergen, Norway. tfan@haukeland.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adjuvants, Anesthesia Anesthesia* Anesthesia, Inhalation Anesthetics, Intravenous Animals Cardioplegic Solutions Cardiopulmonary Bypass* Fentanyl Heart / physiopathology* Heart Arrest, Induced* Hemodynamics / drug effects Hydrocortisone / blood Midazolam Monitoring, Intraoperative Myocardial Contraction / drug effects Myocardial Reperfusion Neuromuscular Blockade* Nitrous Oxide Pentobarbital Research Design Swine Ventricular Function, Left / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Adjuvants, Anesthesia; 0/Anesthetics, Intravenous; 0/Cardioplegic Solutions; 10024-97-2/Nitrous Oxide; 437-38-7/Fentanyl; 50-23-7/Hydrocortisone; 59467-70-8/Midazolam; 76-74-4/Pentobarbital |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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