Document Detail

An amperometric biosensor for uric acid determination prepared from uricase immobilized in polypyrrole film.
MedLine Citation:
PMID:  16809136     Owner:  NLM     Status:  MEDLINE    
In order to prepare a biosensor for the determination of uric acid, electropolymerization of pyrrole on Pt surface was carried out with an electrochemical cell containing pyrrole, ferrocene (as a electron mediator) and tetrabutylammonium tetrafluoroborat in acetonitrile by cyclic voltammetry between 0.0 and 1.0 V (vs. Ag/AgCl) at a scan rate of 50 mV/s upon Pt electrode. Uricase was immobilized by a glutaraldehyde/gelatine croslinking procedure on to polypyrrole film after the electropolymerization processes. The response of the biosensor against uric acid was measured after 330 seconds following the application of a constant potential of +0.7 V (vs. Ag/AgCl). The resulting biosensor exhibits excellent electrocatalysis for the uric acid. The amperometric determination is based on the electrochemical detection of H2O2, which is generated in enzymatic reaction of uric acid. The sensor responds to uric acid with a detection limit of 5.0 x 10(-7) M. The sensor remains relatively stable for 5 weeks. Interference effect were investigated on the amperometric response of the biosensor. Determination of uric acid was carried out in the biological fluids by biosensor.
Servet Cete; Ahmet Yaşar; Fatma Arslan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial cells, blood substitutes, and immobilization biotechnology     Volume:  34     ISSN:  1073-1199     ISO Abbreviation:  Artif Cells Blood Substit Immobil Biotechnol     Publication Date:  2006  
Date Detail:
Created Date:  2006-06-30     Completed Date:  2006-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431307     Medline TA:  Artif Cells Blood Substit Immobil Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  367-80     Citation Subset:  IM    
Department of Chemistry, Faculty of Arts and Sciences, Gazi University, Ankara, Turkey.
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MeSH Terms
Acetaminophen / analysis
Ascorbic Acid / analysis
Biosensing Techniques / methods*
Body Fluids / chemistry
Enzyme Stability
Enzymes, Immobilized
Hydrogen-Ion Concentration
Methionine / analysis
Reproducibility of Results
Urate Oxidase
Uric Acid / analysis*,  blood,  urine
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Polymers; 0/Pyrroles; 103-90-2/Acetaminophen; 30604-81-0/polypyrrole; 50-81-7/Ascorbic Acid; 63-68-3/Methionine; 69-93-2/Uric Acid; EC Oxidase

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