Document Detail


An aminotransferase branch point connects purine catabolism to amino acid recycling.
MedLine Citation:
PMID:  20852637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although amino acids are known precursors of purines, a pathway for the direct recycling of amino acids from purines has never been described at the molecular level. We provide NMR and crystallographic evidence that the PucG protein from Bacillus subtilis catalyzes the transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine. This activity enables soil and gut bacteria to use the animal purine waste as a source of carbon and nitrogen. The reaction catalyzed by (S)-ureidoglycine-glyoxylate aminotransferase (UGXT) illustrates a transamination sequence in which the same substrate provides both the amino group donor and, via its spontaneous decay, the amino group acceptor. Structural comparison and mutational analysis suggest a molecular rationale for the functional divergence between UGXT and peroxisomal alanine-glyoxylate aminotransferase, a fundamental enzyme for glyoxylate detoxification in humans.
Authors:
Ileana Ramazzina; Roberto Costa; Laura Cendron; Rodolfo Berni; Alessio Peracchi; Giuseppe Zanotti; Riccardo Percudani
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Publication Detail:
Type:  Journal Article     Date:  2010-09-19
Journal Detail:
Title:  Nature chemical biology     Volume:  6     ISSN:  1552-4469     ISO Abbreviation:  Nat. Chem. Biol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-19     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231976     Medline TA:  Nat Chem Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  801-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy.
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MeSH Terms
Descriptor/Qualifier:
Alanine / chemistry,  metabolism
Amino Acid Sequence
Amino Acids / chemistry,  metabolism*
Bacillus subtilis / enzymology*
Biocatalysis
Crystallography, X-Ray
Evolution, Molecular
Glycine / analogs & derivatives,  chemistry,  metabolism
Glyoxylates / chemistry,  metabolism
Humans
Magnetic Resonance Spectroscopy
Models, Molecular
Mutation
Phylogeny
Purines / chemistry,  metabolism*
Substrate Specificity
Transaminases / chemistry,  genetics,  metabolism*
Urea / analogs & derivatives,  chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Glyoxylates; 0/Purines; 0/ureidoglycine; 120-73-0/purine; 56-40-6/Glycine; 56-41-7/Alanine; 57-13-6/Urea; EC 2.6.1.-/Transaminases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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