Document Detail


The amino terminus of the adenovirus fiber protein encodes the nuclear localization signal.
MedLine Citation:
PMID:  1962447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using a recombinant vaccinia virus vector, the fiber protein from adenovirus serotype 2 has been expressed in human cells; the protein expressed was correctly assembled into trimers, glycosylated, and transported to the nucleus. Deletion of amino acids 2-5 (KRAR) resulted in accumulation of fiber in the cytoplasm; fusion of the sequence TKRVRL, found at the beginning of Ad7 fiber, to the N-terminus of this mutant restored correct targeting. Changing the charge of amino acids 91 and 92 within another potential targeting sequence (LKKTK to LEETK) had little effect on nuclear targeting. When fused to the N-terminus of beta-galactosidase and expressed in recombinant vaccinia virus, neither MKRARP nor MTKRVRL (from Ad2 and Ad7 fibers, respectively), were sufficient for efficient transport of the hybrid protein to the nucleus; on the other hand, fusions of either MKRARPSEDTF (from Ad2 fiber) or of MKRPRP (a known targeting sequence from the C-terminus of Ad2 E1A proteins) to beta-galactosidase were localized to the nucleus. These results suggest that sequences at the N-terminus of Ad2 and Ad7 fiber are required for correct nuclear targeting.
Authors:
J S Hong; J A Engler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  185     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-01-07     Completed Date:  1992-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  758-67     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, School of Medicine, University of Alabama, Birmingham 35294.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics,  metabolism*
Amino Acid Sequence
Antibodies, Monoclonal
Base Sequence
Capsid / chemistry,  genetics,  metabolism*
Capsid Proteins*
Cell Line
Cell Nucleus / metabolism
Fluorescent Antibody Technique
Gene Expression / physiology
Humans
Immunoblotting
Macromolecular Substances
Molecular Sequence Data
Mutagenesis
Plasmids / genetics
Protein Sorting Signals / genetics,  metabolism*
Recombinant Fusion Proteins / biosynthesis,  genetics,  metabolism
Vaccinia virus / genetics,  metabolism
beta-Galactosidase / genetics
Grant Support
ID/Acronym/Agency:
CA13148/CA/NCI NIH HHS; GM38234/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Capsid Proteins; 0/Macromolecular Substances; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; 0/hexon capsid protein, Adenovirus; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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