| An amino acid in the heptad repeat 1 domain is important for the haemagglutinin-neuraminidase-independent fusing activity of simian virus 5 fusion protein. | |
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MedLine Citation:
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PMID: 10675409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A canine isolate (strain T1) of simian virus 5 (SV-5) performed multiple replication in BHK cells but did not induce cell fusion for up to 3 days. In contrast, a prototype strain (WR) provoked extensive cell fusion within 2 days during the course of its replication. Accordingly, the fusion (F) protein of the T1 strain did not cause cell fusion even when co-expressed with the SV-5 haemagglutinin-neuraminidase (HN) protein, whereas the WR F protein induced cell fusion in the presence of the HN protein. Differences in the predicted amino acid sequences of the T1 and WR F proteins were found at 12 positions and it was proved that the T1 F protein had a longer cytoplasmic tail than the WR F protein. By reducing the length of the cytoplasmic tail or by replacing the tail with the WR F counterpart, the T1 F protein partly restored its HN-dependent fusing activity. Chimeric and mutational analyses between the T1 F protein and the mutant F protein (L22P) suggested that Glu-132 in the heptad repeat 1 domain was involved in the HN-independent fusing activity in addition to the previously identified Pro-22 at the F(2) N terminus. It was also shown that Ala-290 in the heptad repeat 3 domain contributed to the HN-independent fusing activity to some extent. |
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Authors:
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M Ito; M Nishio; H Komada; Y Ito; M Tsurudome |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 81 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2000 Mar |
Date Detail:
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Created Date: 2000-03-27 Completed Date: 2000-03-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 719-27 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Cell Fusion Cell Line Cercopithecus aethiops Cricetinae Dogs Hemagglutinins, Viral / genetics, physiology Molecular Sequence Data Mutagenesis, Site-Directed Neuraminidase / genetics, physiology Protein Structure, Tertiary Recombinant Fusion Proteins / genetics, physiology Respirovirus / genetics, pathogenicity*, physiology Sequence Homology, Amino Acid Vero Cells Viral Fusion Proteins / genetics, physiology* Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Hemagglutinins, Viral; 0/Recombinant Fusion Proteins; 0/Viral Fusion Proteins; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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