| The amino 1H resonances of oligonucleotide helices: d(CGCG). | |
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MedLine Citation:
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PMID: 6400828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An examination of the 1H NMR assignments and exchange properties of the amino resonances of the self-complementary tetramer, d(CGCG) was undertaken with regard to buffer effects, transfer of saturation from the water resonance and temperature dependence of amino 1H line shape and chemical shift. The lack of buffer effect on visible exchangeable proton resonances is evidence for the stringent requirement for nucleo-base protonation at pH values below neutrality, which is greatly reduced in the helical state. For this reason, sharp resonances are observed for both Watson-Crick and non-Watson-Crick cytosine amino protons for base-paired regions. Considerations of monomeric exchange mechanisms for the cytosine and guanine amino protons formed the basis for successful assignment and isolation of their resonances in the helical state by presaturation of the water resonance at selected pH values. Preirradiation of the water resonance at pH less than 6 would isolate the guanine amino 1H resonances of any self-complementary oligonucleotide, to exploit its high sensitivity as a useful proble of helix in equilibrium coil premelting. |
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Authors:
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B McConnell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of biomolecular structure & dynamics Volume: 1 ISSN: 0739-1102 ISO Abbreviation: J. Biomol. Struct. Dyn. Publication Date: 1984 Jun |
Date Detail:
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Created Date: 1990-03-14 Completed Date: 1990-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8404176 Medline TA: J Biomol Struct Dyn Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1407-21 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amines Hydrogen Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Structure Nucleic Acid Conformation* Oligodeoxyribonucleotides* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Oligodeoxyribonucleotides; 1333-74-0/Hydrogen; 68923-10-4/d(CGCG) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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