Document Detail


The ameba Balamuthia mandrillaris feeds by entering into mammalian cells in culture.
MedLine Citation:
PMID:  17910691     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microscopic observations of live cultures of the pathogenic ameba Balamuthia mandrillaris and mammalian cells showed that amebic feeding involved the invasion of the pseudopodia, and/or the whole ameba into the cells. The ameba, recognized by their size and flow of organelles in the cytosol, was seen to extend the tip of a pseudopodium into the cytoplasm of a cell where it moved about leaving visible damage when retracted. In rounded cells, whole amebas were seen to enter into and move around before exiting a cell and then remain quiescent for hours. The invaded mammalian cells retained their turgidity and excluded vital dyes until only their denuded nuclei remained. The cytoplasm of the cells was consumed first, then the nuclei, but not their mitotic chromosomes. The feeding pattern of four isolates of B. mandrillaris, two from humans and two from soil samples, was by amebic invasion into the mammalian cells. The resulting ameba population included cysts, amebas on the surface, and free-floating amebas as individuals or in dense-packed clusters. There was no morphologic indication of a cytopathic change in the mammalian cells before their invasion by the amebas. Feeding by cell invasion is a distinctive feature of B. mandrillaris.
Authors:
Thelma H Dunnebacke
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  54     ISSN:  1066-5234     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-10-03     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  452-64     Citation Subset:  IM    
Affiliation:
California Department of Health Services, Viral and Rickettsial Disease Laboratory, Richmond, California 94804-6403, USA. dunnebacke@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cell Line
Cricetinae
Cricetulus
Humans
Lobosea / cytology*,  physiology*
Male
Microscopy, Phase-Contrast
Pseudopodia / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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