Document Detail


An alternatively spliced isoform of B-Myb is a transcriptional inhibitor.
MedLine Citation:
PMID:  11114719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
B-Myb is a highly conserved member of the Myb transcription factor family. The primary transcript of the B-myb gene is spliced alternatively in two mRNAs which either contain or lack a sequence corresponding to the so-called exon 9A of c-myb. Recent studies showed that full-length B-Myb containing the exon 9A encoded amino acids is a cell cycle regulated transcription factor whose activity is stimulated by cyclin A/Cdk 2-dependent phosphorylation at the carboxyl-terminus of B-Myb. We have now investigated in more detail the transactivation potential of the shorter isoform of B-Myb lacking exon 9A. Here, we show that B-Myb lacking exon 9A has no transactivation activity even in the presence of cyclin A. This inactivity of the shorter isoform of B-Myb is not due an improper subcelluar localization. Our work suggests that B-Myb lacking exon 9A may act as an inhibitor for full-length B-Myb mediated transactivation. Furthermore, by analysing the transactivation potential of Gal4/B-Myb fusion proteins we have identified the amino-terminal part of the exon 9A as the principal transactivation domain of full-length B-Myb. The results presented here demonstrate that B-myb encodes both an activator and an inhibitor of transcription and, thus, reveal an additional level of regulation of B-Myb activity beside the known cyclin dependent mechanisms.
Authors:
S Horstmann; S Ferrari; K H Klempnauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  19     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-20     Completed Date:  2000-12-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  5428-34     Citation Subset:  IM    
Affiliation:
Institut für Biochemie, Westfälische-Wilhelms-Universität Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Amino Acid Sequence
Animals
Cell Cycle Proteins*
Cell Nucleus / metabolism
Chickens
Conserved Sequence
Cyclin A / physiology
DNA-Binding Proteins / genetics*,  metabolism
Exons
Genes, Reporter
Humans
Mice
Molecular Sequence Data
Peptide Mapping
Protein Isoforms
Protein Structure, Tertiary
RNA, Messenger / genetics,  metabolism
Sequence Homology, Amino Acid
Trans-Activators / genetics*,  metabolism
Transcription, Genetic / physiology*
Transcriptional Activation / physiology*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclin A; 0/DNA-Binding Proteins; 0/MYBL2 protein, human; 0/Mybl2 protein, mouse; 0/Protein Isoforms; 0/RNA, Messenger; 0/Trans-Activators

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