Document Detail

An alternative thermodilution method to measure cardiac output in a rat model of stagnant hypoxia.
MedLine Citation:
PMID:  11092688     Owner:  NLM     Status:  MEDLINE    
Rats are often used to study hemodynamics in animal research. We have established an alternative method to measure cardiac output in a conscious rat using a thermodilution technique via a left ventricular injection. The validity of this method was evaluated in conscious rats and compared with the results obtained using the radiolabeled microspheres (reference sample method). Using 20 male Sprague-Dawley rats, a baseline cardiac index was measured by thermodilution to determine the baseline cardiac index and to evaluate between animal variability. The baseline cardiac index was compared to the reference sample method with 6 rats. Following baseline measurements, an intra-atrial balloon was inflated in a stepwise manner to create 2 to 3 different cardiac outputs, and the cardiac index was computed. For each measurement, the cardiac index was first measured by thermodilution and immediately followed by the reference sample method. A total of 21 measurements were obtained, and the results were analyzed by a Bland-Altman plot and the correlation coefficient was calculated. Although the agreement between the two methods was poor, both methods had a good correlation (r2 = 0.59). With the thermodilution technique, we demonstrated a small coefficient of variation in each measurement, with a low intra-animal and inter-animal variability. As there is no gold standard method to measure cardiac output in rats, we believe that left ventricular thermodilution is a reliable method, and overcomes several technical difficulties such as heat loss, one of the significant limitations of the conventional thermodilution method (via right atrial injection). This new thermodilution technique (via the left ventricle) is therefore an attractive alternative method to measure cardiac output in rats.
Y Morita; P Yu; C M Martin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  14     ISSN:  1073-2322     ISO Abbreviation:  Shock     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-03-06     Completed Date:  2001-05-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  550-4     Citation Subset:  IM    
AC Burton Vascular Biology Laboratory, London Health Sciences Centre and University of Western Ontario, Ontario, Canada.
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MeSH Terms
Anoxia / physiopathology*
Blood Pressure
Cardiac Output / physiology*
Cardiac Output, Low / physiopathology*
Disease Models, Animal
Heart Rate
Hemodynamics / physiology*
Rats, Sprague-Dawley
Thermodilution / methods
Ventricular Function, Left

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