Document Detail


An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections.
MedLine Citation:
PMID:  7983365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff-like reagent. Conditions for obtaining specific and sensitive Feulgen-like staining are determined.
Authors:
V T Mikhaylova; D V Markov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  42     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-05     Completed Date:  1995-01-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1643-9     Citation Subset:  IM    
Affiliation:
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
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MeSH Terms
Descriptor/Qualifier:
Acrylic Resins
Animals
DNA / analysis*
Indicators and Reagents*
Liver / chemistry*,  ultrastructure
Male
Osmium Compounds*
Quaternary Ammonium Compounds*
Rats
Rats, Wistar
Sulfhydryl Compounds*
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Indicators and Reagents; 0/Osmium Compounds; 0/Quaternary Ammonium Compounds; 0/Sulfhydryl Compounds; 0/osmium ammine; 507-09-5/thioacetic acid; 84137-04-2/Lowicryl 4KM resin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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