| An alternative method for intrathymic injections in mice. | |
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MedLine Citation:
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PMID: 20664574 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The thymus is a bi-lobed lymphatic organ located in the anterior portion of the ventral thoracic cavity, just behind the sternum. Because the thymus is the site of development of T lymphocytes (T cells), it is frequently targeted in research studies that involve the immune system. Furthermore, the rapid expansion of transgenic and gene-targeted mouse models of immune disorders has enabled a concomitant increase in the number of studies of T-cell development. Such studies may require the administration of intrathymic injections, which have traditionally been done using a surgical approach. Surgical manipulation can result in pain or distress to the animal, which may affect the immune system, potentially confounding experimental results. Here, the authors describe a nonsurgical, ultrasound-guided approach for intrathymic injection in the mouse that results in negligible distress to the animal. |
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Authors:
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Robin Blair-Handon; Kristen Mueller; Shelley Hoogstraten-Miller |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Lab animal Volume: 39 ISSN: 0093-7355 ISO Abbreviation: Lab Anim (NY) Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-09-16 Revised Date: 2012-03-08 |
Medline Journal Info:
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Nlm Unique ID: 0417737 Medline TA: Lab Anim (NY) Country: United States |
Other Details:
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Languages: eng Pagination: 248-52 Citation Subset: IM |
Affiliation:
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Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Injections / methods, veterinary* Mice Reproducibility of Results Surgical Procedures, Minimally Invasive / methods, veterinary* Thymus Gland* |
| Grant Support | |
ID/Acronym/Agency:
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Z99 HG999999/HG/NHGRI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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