Document Detail

An alternative method for the determination of uric acid in serum.
MedLine Citation:
PMID:  241514     Owner:  NLM     Status:  MEDLINE    
A novel fluorometric method for the determination of uric acid based on the coupled reactions of uric acid with uricase and peroxidase to form highly fluorescent 2,2'dihydroxy-3,3'-dimethoxy-biphenyl-5,5'-diacetic acid is described. The calibration curve was constructed from a series of standard uric acid solutions vs. the corresponding relative fluorescence. It was linear up to 15 mg/dl (0.9 mmol/l). The serum or plasma samples must be deproteinated with (absolute) ethanol before assay and its uric acid content can be obtained from the calibration curve. This method is rather simple, having good precision and accuracy. High level ascorbic acid in the sample falsely elevates uric acid concentration. Comparison of the results obtained on the patient sera with a colorimetric phosphotungstate method and a standard enzymatic spectrophotometric method gave coefficients of correlation of 0.908 and 0.986, respectively.
J C Kuan; S S Kuan; G G Guilbault
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  64     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1976-02-02     Completed Date:  1976-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  19-25     Citation Subset:  IM    
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MeSH Terms
Ascorbic Acid / blood
Horseradish Peroxidase
Hydrogen-Ion Concentration
Kidney Diseases / blood
Renal Dialysis
Spectrometry, Fluorescence
Urate Oxidase
Uric Acid / blood*
Reg. No./Substance:
50-81-7/Ascorbic Acid; 69-93-2/Uric Acid; EC 1.11.1.-/Horseradish Peroxidase; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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